ARG21553
Goat anti-Mouse Kappa Light Chain antibody (Biotin), pre-adsorbed
Goat anti-Mouse Kappa Light Chain antibody (Biotin), pre-adsorbed for ELISA,ELISPOT,Flow cytometry,FLISA,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Mouse
Overview
| Product Description | Biotin-conjugated Goat Polyclonal antibody recognizes Mouse Kappa Light Chain |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | ELISA, ELISPOT, FACS, FLISA, ICC/IF, IHC-Fr, IHC-P, IP, WB |
| Specificity | The antibody reacts with Mouse kappa light chains. The antibody is pre-adsorbed with Mouse lambda light chains, so the antibody may not react with Mouse lambda light chains, but may react with kappa light chains from other species. |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Kappa Light Chain |
| Antigen Species | Mouse |
| Conjugation | Biotin |
Application Instructions
| Pre Adsorbed | Mouse lambda light chains. | ||||||||||||||||||||
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| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS and 0.1% Sodium azide. |
| Preservative | 0.1% Sodium azide |
| Concentration | 0.5 mg/ml |
| Storage Instruction | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Images (4) Click the Picture to Zoom In
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ARG21553 Goat anti-Mouse Kappa Light Chain antibody (Biotin) (pre-adsorbed) ICC/IF image
Immunofluorescence: Human pancreatic carcinoma cell line MIA PaCa-2 stained with anti-Cytokeratin 18 antibody (right), followed by ARG21553 Goat anti-Mouse Kappa Light Chain antibody (Biotin) (pre-adsorbed), Streptavidin (FITC) and DAPI.
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ARG21553 Goat anti-Mouse Kappa Light Chain antibody (Biotin) (pre-adsorbed) ELISA image
ELISA: The plate was coated with purified Mouse IgGκ, IgMκ, IgAκ, IgGλ, IgMλ, and IgAλ. Immunoglobulins were detected with serially diluted ARG21553 Goat anti-Mouse Kappa Light Chain antibody (Biotin) (pre-adsorbed) followed by ARG23912 Streptavidin (HRP).
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ARG21553 Goat anti-Mouse Kappa Light Chain antibody (Biotin) (pre-adsorbed) FACS image
Flow Cytometry: BALB/c Mouse splenocytes stained with ARG21553 Goat anti-Mouse Kappa Light Chain antibody (Biotin) (pre-adsorbed) followed by Streptavidin (FITC).
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ARG21553 Goat anti-Mouse Kappa Light Chain antibody (Biotin) (pre-adsorbed) ICC/IF image
Immunofluorescence: Human pancreatic carcinoma cell line MIA PaCa-2 stained with anti-Cytokeratin 18 antibody (right), followed by ARG21553 Goat anti-Mouse Kappa Light Chain antibody (Biotin) (pre-adsorbed), Streptavidin (FITC) and DAPI.
