ARG70597
Human CD72 recombinant protein (His-tagged, C-ter)
Human CD72 recombinant protein (His-tagged, C-ter) for SDS-PAGE
Overview
| Product Description | CHO expressed, His-tagged (C-ter) Human CD72 recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | CD72 |
| Species | Human |
| A.A. Sequence | Arg117 - Asp359 |
| Expression System | CHO |
| Alternate Names | CD72; CD72 molecule; CD antigen CD72; LYB2; B-cell differentiation antigen CD72; Lyb-2; CD72b |
Properties
| Form | Powder |
|---|---|
| Purification Note | Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 85% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | For long term, lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | CD72 |
|---|---|
| Gene Full Name | CD72 molecule |
| Background | CD72 is a transmembrane glycoprotein expressed as a homodimer especially in B cells, but also in other antigen presenting cells such as dendritic cells and macrophages. Through one of its immunoreceptor tyrosine-based inhibitory motives (ITIMs), CD72 interacts with tyrosine phosphatase SHP-1, thereby suppressing B cell responsiveness. Binding of CD72 with its ligand CD100 (Sema4D) prevents BCR association and phosphorylation of CD72 and results in dissociation of SHP-1 from CD72, thus enables B cell activation. |
| Function | Plays a role in B-cell proliferation and differentiation. [UniProt] |
| Cellular Localization | Membrane. [UniProt] |
| PTM | Phosphorylated upon engagement of the B-cell receptor, probably by LYN or SYK. Phosphorylation at Tyr-7 is important for interaction with PTPN6/SHP-1 (By similarity). [UniProt] |
