ARG70142
Human CNTF recombinant protein (Active) (His-tagged, C-ter)
Human CNTF recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE
Overview
| Product Description | E. coli expressed, His-tagged (C-ter) Active Human CNTF recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | CNTF |
| Species | Human |
| A.A. Sequence | Met1 - Met200 |
| Expression System | E. coli |
| Activity | Active |
| Activity Note | Determined by its ability to induce proliferation in TF-1 cells. The ED50 for this effect is < 0.15 μg/mL. |
| Alternate Names | HCNTF; CNTF; Ciliary neurotrophic factor |
Properties
| Form | Powder |
|---|---|
| Purification Note | Endotoxin level is < 0.01 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 98% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | For long term, lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | CNTF |
|---|---|
| Gene Full Name | ciliary neurotrophic factor |
| Background | The protein encoded by this gene is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. The protein is a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. A mutation in this gene, which results in aberrant splicing, leads to ciliary neurotrophic factor deficiency, but this phenotype is not causally related to neurologic disease. A read-through transcript variant composed of the upstream ZFP91 gene and CNTF sequence has been identified, but it is thought to be non-coding. Read-through transcription of ZFP91 and CNTF has also been observed in mouse. [provided by RefSeq, Oct 2010] |
| Function | CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy. [UniProt] |
| Cellular Localization | Cytoplasm. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG70142 Human CNTF recombinant protein (Active) (His-tagged, C-ter) SDS-PAGE image
SDS-PAGE analysis of ARG70142 Human CNTF recombinant protein (Active) (His-tagged, C-ter).
