ARG70579
Human Tissue Factor recombinant protein (His-tagged, C-ter)
Human Tissue Factor recombinant protein (His-tagged, C-ter) for SDS-PAGE
Overview
| Product Description | CHO expressed, His-tagged (C-ter) Human Tissue Factor recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | Tissue Factor |
| Species | Human |
| A.A. Sequence | Ser33 - Glu251 |
| Expression System | CHO |
| Alternate Names | F3; coagulation factor III (thromboplastin, tissue factor); Thromboplastin; Tissue factor; TFA; CD142; TF; Coagulation factor III; CD antigen CD142 |
Properties
| Form | Powder |
|---|---|
| Purification Note | Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | For long term, lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | F3 |
|---|---|
| Gene Full Name | coagulation factor III (thromboplastin, tissue factor) |
| Background | This gene encodes coagulation factor III which is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII. The resulting complex provides a catalytic event that is responsible for initiation of the coagulation protease cascades by specific limited proteolysis. Unlike the other cofactors of these protease cascades, which circulate as nonfunctional precursors, this factor is a potent initiator that is fully functional when expressed on cell surfaces. There are 3 distinct domains of this factor: extracellular, transmembrane, and cytoplasmic. This protein is the only one in the coagulation pathway for which a congenital deficiency has not been described. Alternate splicing results in multiple transcript variants.[provided by RefSeq, May 2010] |
| Function | Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited protolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade. [UniProt] |
| Cellular Localization | Membrane; Secreted. [UniProt] |
| Highlight | Related products: Tissue Factor antibodies; Tissue Factor ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | Disulfide bond; Glycoprotein; Lipoprotein; Palmitate. [UniProt] |
