ARG83378
Pyrophosphate Assay Kit
Pyrophosphate Assay Kit for Functional study and Other
Pyrophosphates, Anions, Dietary minerals, Molecular biology, Nucleotides, E-number additives
Overview
| Product Description | ARG83378 Pyrophosphate Assay Kit is an Assay kit for the quantification of Pyrophosphate in Serum, Plasma, Saliva, Urine and Cell / Tissue lysates |
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| Tested Reactivity | Other |
| Tested Application | FuncSt |
| Target Name | Pyrophosphate |
| Conjugation | Un-conjugated |
| Conjugation Note | Read at 590 nm. |
| Sensitivity | 2.5 µM |
| Sample Type | Serum, Plasma, Saliva, Urine and Cell / Tissue lysates |
| Standard Range | 3.91 - 125 µM |
| Sample Volume | 50 µL |
Application Instructions
| Assay Time | ~30 min |
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Properties
| Form | Liquid |
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| Storage Instruction | Store components at -20°C, -80°C. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Background | Pyrophosphate is a potent inhibitor of calcium-phosphate crystal formation and growth. Extracellular pyrophosphate is produced upon ATP hydrolysis by the enzyme ectonucleotide pyrophosphatase/phosphodiesterase 1 (eNPP1). Pyrophosphate is degraded to phosphate by tissue-nonspecific alkaline phosphatase (TNAP), promoting calcification. Another enzyme, ectonucleoside triphosphate diphosphohydrolase 1 (eNTPD1), can hydrolyze ATP and ADP to phosphate, reducing the availability of ATP for pyrophosphate production and likely inducing calcification. |
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| Function | The anion P2O7 is abbreviated PPi, standing for inorganic pyrophosphate. It is formed by the hydrolysis of ATP into AMP in cells. |
| Research Area | Pyrophosphates, Anions, Dietary minerals, Molecular biology, Nucleotides, E-number additives |
Images (1) Click the Picture to Zoom In
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ARG83378 Pyrophosphate Assay Kit standard curve image
ARG83378 Pyrophosphate Assay Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
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| ARG83378 Pyrophosphate Assay Kit User manual |
 Download
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