ARG70218
SARS-CoV-2 Spike recombinant protein (S1 Subunit) (His-tagged, C-ter)
SARS-CoV-2 Spike recombinant protein (S1 Subunit) (His-tagged, C-ter) for SDS-PAGE
Publication12
Overview
| Product Description | E. coli expressed, His-tagged (C-ter) SARS-CoV-2 Spike recombinant protein (S1 Subunit) |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | SARS-CoV-2 Spike protein (S1 Subunit) |
| Species | Virus |
| A.A. Sequence | Val16 - Cys671 |
| Expression System | E. coli |
Properties
| Form | Powder |
|---|---|
| Purity | > 98% (by SDS-PAGE) |
| Buffer | PBS (pH 8.0) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not <200 μg/mL and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | For long term, lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Highlight | Related products: SARS-CoV antibodies; SARS-CoV ELISA Kits; SARS-CoV recombinant proteins; Related news: HMGB1, a biomarker and therapeutic target in COVID-19 Exploring Antiviral Immune Response |
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ARG70218 SARS-CoV-2 Spike recombinant protein (S1 Subunit) (His-tagged, C-ter) SDS-PAGE image
SDS-PAGE analysis of ARG70218 SARS-CoV-2 Spike recombinant protein (S1 Subunit) (His-tagged, C-ter).
Specific References
Cytokines from SARS-CoV-2 Spike-Activated Macrophages Hinder Proliferation and Cause Cell Dysfunction in Endothelial Cells
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Cytokine-Induced iNOS in A549 Alveolar Epithelial Cells: A Potential Role in COVID-19 Lung Pathology
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Detoxified synthetic bacterial membrane vesicles as a vaccine platform against bacteria and SARS-CoV-2
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Growth Arrest of Alveolar Cells in Response to Cytokines from Spike S1-Activated Macrophages: Role of IFN-γ
Functional assay /
The JAK1/2 Inhibitor Baricitinib Mitigates the Spike-Induced Inflammatory Response of Immune and Endothelial Cells In Vitro
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SARS-CoV-2 Spike protein is not pro-inflammatory in human primary macrophages: endotoxin contamination and lack of protein glycosylation as possible confounders
Functional Assay /
Immune-Mediated Inflammatory Responses of Alveolar Epithelial Cells: Implications for COVID-19 Lung Pathology
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In Vitro Exposure of Primary Human T Cells and Monocytes to Polyclonal Stimuli Reveals a Basal Susceptibility to Display an Impaired Cellular Immune Response and Develop Severe COVID-19
Functional assay /
Standardized Extract of Asparagus officinalis Stem Attenuates SARS-CoV-2 Spike Protein-Induced IL-6 and IL-1β Production by Suppressing p44/42 MAPK and Akt Phosphorylation in Murine Primary Macrophages
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Endothelial Cell Activation by SARS-CoV-2 Spike S1 Protein: A Crosstalk between Endothelium and Innate Immune Cells
Functional assay /
ETAS®50 Attenuates SARS-CoV-2 Spike Protein-Induced IL-6 and IL-1β Production by Suppressing p44/42 MAPK and Akt Phosphorylation in Murine Primary Macrophages.
Functional assay /
SARS-CoV-2 spike protein S1 subunit induces pro-inflammatory responses via toll-like receptor 4 signaling in murine and human macrophages
Functional assay /
