ARG82166
alpha Glucosidase Activity Assay Kit (Colorimetric)
alpha Glucosidase Activity Assay Kit (Colorimetric) for Functional study and Human,Mouse,Rat,All
Publication1
Overview
| Product Description | ARG82166 alpha Glucosidase Activity Assay Kit (Colorimetric) can be used to measure alpha Glucosidase activity in biological samples. |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, All |
| Tested Application | FuncSt |
| Target Name | alpha Glucosidase |
| Conjugation Note | Read at 405 nm |
| Sensitivity | 2 U/L |
| Detection Range | 2 - 250 U/L |
| Sample Type | biological samples |
| Sample Volume | 20 µl |
| Alternate Names | Lysosomal alpha-glucosidase; LYAG; Aglucosidase alfa; Acid maltase; EC 3.2.1.20 |
Application Instructions
| Application Note | Please note that this kit does not include a microplate. |
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| Assay Time | 20 min |
Properties
| Form | Liquid |
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| Storage Instruction | Store the kit at -20°C. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | GAA |
| Gene Full Name | glucosidase, alpha; acid |
| Background | This gene encodes acid alpha-glucosidase, which is essential for the degradation of glycogen to glucose in lysosomes. Different forms of acid alpha-glucosidase are obtained by proteolytic processing. Defects in this gene are the cause of glycogen storage disease II, also known as Pompe's disease, which is an autosomal recessive disorder with a broad clinical spectrum. Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008] |
| Function | Essential for the degradation of glygogen to glucose in lysosomes. [UniProt] |
| Cellular Localization | Lysosome. Lysosome membrane. [UniProt] |
| PTM | The different forms of acid glucosidase are obtained by proteolytic processing. Phosphorylation of mannose residues ensures efficient transport of the enzyme to the lysosomes via the mannose 6-phosphate receptor. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG82166 alpha Glucosidase Activity Assay Kit (Colorimetric) enzyme kinetics graph
Kinetics of 0 and 250 U/L alpha Glucosidase reaction, using ARG82166 alpha Glucosidase Activity Assay Kit (Colorimetric).
Specific References
Anti-Obesity Effects of Sorbi Lignum et Fructus in High-Fat Diet-Induced Obese Mice
FuncSt / Mouse / Serum
| Title | Download Link |
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| ARG82166 alpha Glucosidase Activity Assay Kit (Colorimetric) User manual |
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