ARG65422
anti-5-bromodeoxyuridine / BrdU antibody [Bu20a]
anti-5-bromodeoxyuridine / BrdU antibody [Bu20a] for Flow cytometry,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections and Other
Cell Biology and Cellular Response antibody; Controls and Markers antibody; Gene Regulation antibody; Neuroscience antibody
Overview
| Product Description | Mouse Monoclonal antibody [Bu20a] recognizes 5-bromodeoxyuridine / BrdU |
|---|---|
| Tested Reactivity | Other |
| Tested Application | FACS, ICC/IF, IHC-Fr, IHC-P |
| Specificity | The clone Bu20a reacts specifically with BrdU incorporated into DNA during S-phase of a cell cycle. It is useful for detecting proliferating cells by flow cytometry or immunohistochemistry staining. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | Bu20a |
| Isotype | IgG1 |
| Target Name | 5-bromodeoxyuridine / BrdU |
| Immunogen | Bromodeoxyuridine conjugated to BSA |
| Conjugation | Un-conjugated |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from cell culture supernatant by protein-A affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Background | Bromodexyuridine (BrdU) is a thymidine analog which is selectively incorporated into the DNA of proliferating cells to provide a marker for the DNA being replicated. The number of proliferating cells can then be detected in cell lysates, tissue sections or suspensions using an antibody specific for the BrdU. |
|---|---|
| Research Area | Cell Biology and Cellular Response antibody; Controls and Markers antibody; Gene Regulation antibody; Neuroscience antibody |
Clone References
Presentation of fibronectin fragments using affinity protein interactions for enhanced retention and function.
Double immunocytochemical labeling of cell and tissue samples with monoclonal anti-bromodeoxyuridine.
ICC/IF / Human, Mouse
