ARG54229
anti-5-bromodeoxyuridine / BrdU antibody [Bu20a] (PE)
anti-5-bromodeoxyuridine / BrdU antibody [Bu20a] (PE) for Flow cytometry and Other
Cell Biology and Cellular Response antibody; Controls and Markers antibody; Gene Regulation antibody; Neuroscience antibody
Overview
| Product Description | PE-conjugated Mouse Monoclonal antibody [Bu20a] recognizes 5-bromodeoxyuridine / BrdU |
|---|---|
| Tested Reactivity | Other |
| Tested Application | FACS |
| Specificity | The clone Bu20a reacts specifically with BrdU incorporated into DNA during S-phase of a cell cycle. It is useful for detecting proliferating cells by flow cytometry or immunohistochemistry staining. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | Bu20a |
| Isotype | IgG1 |
| Target Name | 5-bromodeoxyuridine / BrdU |
| Immunogen | Bromodeoxyuridine conjugated to BSA |
| Conjugation | PE |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification Note | The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary. |
| Buffer | PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA |
| Preservative | 15 mM Sodium azide |
| Stabilizer | 0.2% (w/v) high-grade protease free BSA |
| Storage Instruction | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Background | Bromodexyuridine (BrdU) is a thymidine analog which is selectively incorporated into the DNA of proliferating cells to provide a marker for the DNA being replicated. The number of proliferating cells can then be detected in cell lysates, tissue sections or suspensions using an antibody specific for the BrdU. |
|---|---|
| Research Area | Cell Biology and Cellular Response antibody; Controls and Markers antibody; Gene Regulation antibody; Neuroscience antibody |
Images (1) Click the Picture to Zoom In
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ARG54229 anti-5-bromodeoxyuridine / BrdU antibody [Bu20a] (PE) FACS image
Flow Cytometry: BrdU incorporated into the K562 cells. Cells were stained with ARG54229 anti-5-bromodeoxyuridine / BrdU antibody [Bu20a] (PE).
Clone References
Presentation of fibronectin fragments using affinity protein interactions for enhanced retention and function.
Double immunocytochemical labeling of cell and tissue samples with monoclonal anti-bromodeoxyuridine.
ICC/IF / Human, Mouse
