ARG41099
anti-ACE2 antibody
anti-ACE2 antibody for IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat
Publication1
Overview
| Product Description | Rabbit Polyclonal antibody recognizes ACE2 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | IHC-P, IP, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | ACE2 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide derived from Human ACE2. |
| Conjugation | Un-conjugated |
| Alternate Names | Angiotensin-converting enzyme homolog; ACEH; Angiotensin-converting enzyme 2; Metalloprotease MPROT15; ACE-related carboxypeptidase; EC 3.4.17.23 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Observed Size | ~ 105 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ACE2 |
| Gene Full Name | angiotensin I converting enzyme 2 |
| Background | The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. The organ- and cell-specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronaviruses SARS and HCoV-NL63. [provided by RefSeq, Jul 2008] |
| Function | Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses. [UniProt] |
| Cellular Localization | Processed angiotensin-converting enzyme 2: Secreted. Cell membrane; Single-pass type I membrane protein. Cytoplasm. Note=Detected in both cell membrane and cytoplasm in neurons. [UniProt] |
| Highlight | Related products: ACE2 antibodies; ACE2 ELISA Kits; ACE2 recombinant proteins; Anti-Rabbit IgG secondary antibodies; Related news: HMGB1, a biomarker and therapeutic target in COVID-19 ACE2, receptor of 2019-nCoV |
| Calculated MW | 92 kDa |
| PTM | N-glycosylation on Asn-90 may limit SARS infectivity. Proteolytic cleavage by ADAM17 generates a secreted form. Also cleaved by serine proteases: TMPRSS2, TMPRSS11D and HPN/TMPRSS1. [UniProt] |
Images (3) Click the Picture to Zoom In
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ARG41099 anti-ACE2 antibody WB image
Western blot: 20 µg of Mouse brain lysate stained with ARG41099 anti-ACE2 antibody at 1:1000 dilution.
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ARG41099 anti-ACE2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human kidney tissue stained with ARG41099 anti-ACE2 antibody.
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ARG41099 anti-ACE2 antibody WB image
Western blot: Human kidney lysate stained with ARG41099 anti-ACE2 antibody.
Customer's Feedback
Excellent
Review for anti-ACE2 antibody
Application：WB
Sample：Mouse brain
Sample Loading Amount：20 μg
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Specific References
Antigenic Cross-Reactivity Between SARS-CoV-2 S1-RBD and Its Receptor ACE2
WB / Mouse
