ARG63492
anti-ACTR1B / ARP1B antibody
anti-ACTR1B / ARP1B antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes ACTR1B / ARP1B |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat, Cow, Dog |
| Tested Application | IHC-P, WB |
| Specificity | This epitope was selected to minimise the chance of cross-reactivity with ACTR1A (YEEDGARSIHRKT). |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | ACTR1B / ARP1B |
| Antigen Species | Human |
| Immunogen | C-YEEDGSRAIHRKT |
| Conjugation | Un-conjugated |
| Alternate Names | PC3; Actin-related protein 1B; CTRN2; Beta-centractin; ARP1B |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by antigen affinity chromatography. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | This gene encodes a 42.3 kD subunit of dynactin, a macromolecular complex consisting of 10 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein and is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit, like ACTR1A, is an actin-related protein. These two proteins, which are of equal length and share 90% amino acid identity, are present in a constant ratio of approximately 1:15 in the dynactin complex. [provided by RefSeq, Aug 2008] |
| Research Area | Signaling Transduction antibody |
| Calculated MW | 42 kDa |
Images (2) Click the Picture to Zoom In
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ARG63492 anti-ACTR1B / ARP1B antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human uterus tissue. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG63492 anti-ACTR1B / ARP1B antibody at 3.8 µg/ml dilution followed by AP-staining.
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ARG63492 anti-ACTR1B / ARP1B antibody WB image
Western blot: Human heart lysate (RIPA buffer, 35 µg total protein per lane) stained with ARG63492 anti-ACTR1B / ARP1B antibody at 1 µg/ml dilution.
