ARG63485
anti-AIRE antibody
anti-AIRE antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Gene Regulation antibody; Immune System antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes AIRE |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, ICC/IF, IHC-P, WB |
| Specificity | This antibody is expected to recognize isoforms 1 and 2 (NP_000374.1 and NP_000649.1 respecively). |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | AIRE |
| Antigen Species | Human |
| Immunogen | C-QSMARPAAPFPS |
| Conjugation | Un-conjugated |
| Alternate Names | APECED; Autoimmune regulator; APS1; APSI; Autoimmune polyendocrinopathy candidiasis ectodermal dystrophy protein; AIRE1; PGA1; APECED protein |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: Steam tissue section in Tris/EDTA buffer (pH 9.0). WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by antigen affinity chromatography. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | This gene encodes a transcriptional regulator that forms nuclear bodies and interacts with the transcriptional coactivator CREB binding protein. The encoded protein plays an important role in immunity by regulating the expression of autoantigens and negative selection of autoreactive T-cells in the thymus. Mutations in this gene cause the rare autosomal-recessive systemic autoimmune disease termed autoimmune polyendocrinopathy with candidiasis and ectodermal dystrophy (APECED). [provided by RefSeq, Jun 2012] |
| Research Area | Gene Regulation antibody; Immune System antibody |
| Calculated MW | 58 kDa |
| PTM | Phosphorylated. Phosphorylation could trigger oligomerization. |
Images (6) Click the Picture to Zoom In
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ARG63485 anti-AIRE antibody WB image
Western Blot: Human Lymph Node lysate (35 µg protein in RIPA buffer) stained with ARG63485 anti-AIRE (isoforms 1 and 2) antibody at 0.5 µg/ml dilution.
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ARG63485 anti-AIRE antibody ICC/IF image
Immunofluorescence: Paraformaldehyde fixed U2OS cells permeabilized with 0.15% Triton. Cells were stained with ARG63485 anti-AIRE antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Phalloidin (red) for Actin filaments staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.
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ARG63485 anti-AIRE antibody IHC-P image
Immunohistochemistry: paraffin embedded Human Thymus. (Steamed antigen retrieval with Tris/EDTA buffer pH 9) stained with ARG63485 anti-AIRE (isoforms 1 and 2) antibody at 2 µg/ml dilution followed by HRP-staining.
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ARG63485 anti-AIRE antibody WB image
Western blot: 35 µg of Jurkat nucleus (A), Human spleen (B) and Human smooth muscle (C, negative control) lysates (in RIPA buffer) stained with ARG63485 anti-AIRE antibody at 1 µg/ml (A) and 2 µg/ml (B, C) dilutions and incubated at RT for 1 hour.
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ARG63485 anti-AIRE antibody FACS image
Flow Cytometry: Paraformaldehyde-fixed Jurkat cells permeabilized with 0.5% Triton. Cells were stained with ARG63485 anti-AIRE antibody (blue line) at 10 µg/ml dilution and overnight, followed by incubation with Alexa FluorR 488 labelled secondary antibody. IgG control: Unimmunized goat IgG (black line).
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ARG63485 anti-AIRE antibody ICC/IF image
Immunofluorescence: Paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton. Cells were stained with ARG63485 anti-AIRE antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Phalloidin (red) for Actin filaments staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.
