ARG40814
anti-AKR1C1 + AKR1C2 antibody
anti-AKR1C1 + AKR1C2 antibody for Western blot and Human,Mouse
Overview
| Product Description | Rabbit Polyclonal antibody recognizes AKR1C1 + AKR1C2 |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | WB |
| Specificity | This antibody might also react to AKR1C3 and AKR1C4 based on sequence homology analysis (>80%). |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | AKR1C1 + AKR1C2 |
| Antigen Species | Human |
| Immunogen | Recombinant fusion protein corresponding to aa. 1-323 of Human AKR1C1 (NP_001344.2). |
| Conjugation | Un-conjugated |
| Alternate Names | 20-alpha-hydroxysteroid dehydrogenase; Trans-1,2-dihydrobenzene-1,2-diol dehydrogenase; EC 1.1.1.112; EC 1.1.1.-; 20-alpha-HSD; Chlordecone reductase homolog HAKRC; DD1; HBAB; C9; DD1/DD2; High-affinity hepatic bile acid-binding protein; Indanol dehydrogenase; 20-ALPHA-HSD; Aldo-keto reductase family 1 member C1; DDH1; EC 1.1.1.149; H-37; MBAB; EC 1.3.1.20; DDH; Dihydrodiol dehydrogenase 1/2; HAKRC; 2-ALPHA-HSD |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| Positive Control | 22Rv1 | ||||
| Observed Size | 37 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q04828 Human Aldo-keto reductase family 1 member C1 |
|---|---|
| Gene Symbol | AKR1C1 |
| Gene Full Name | aldo-keto reductase family 1, member C1 |
| Background | This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the reaction of progesterone to the inactive form 20-alpha-hydroxy-progesterone. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14. [provided by RefSeq, Jul 2008] |
| Function | Converts progesterone to its inactive form, 20-alpha-dihydroxyprogesterone (20-alpha-OHP). In the liver and intestine, may have a role in the transport of bile. May have a role in monitoring the intrahepatic bile acid concentration. Has a low bile-binding ability. May play a role in myelin formation. [UniProt] |
| Cellular Localization | Cytoplasm. [UniProt] |
| Calculated MW | 37 kDa |
Images (1) Click the Picture to Zoom In
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ARG40814 anti-AKR1C1 + AKR1C2 antibody WB image
Western blot: 25 µg of 22Rv1 cell lysate stained with ARG40814 anti-AKR1C1 + AKR1C2 antibody at 1:3000 dilution.
