ARG54128
anti-AMPK beta 1 antibody
anti-AMPK beta 1 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat,Monkey
Cancer antibody; Cell Biology and Cellular Response antibody; Metabolism antibody; Signaling Transduction antibody
Overview
| Product Description | Mouse Monoclonal antibody recognizes PRKAB1 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Mk |
| Tested Application | ICC/IF, IHC-P, IP, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Target Name | AMPK beta 1 |
| Antigen Species | Human |
| Immunogen | Purified recombinant human AMPK beta 1 protein fragments expressed in E.coli. |
| Conjugation | Un-conjugated |
| Alternate Names | AMPKb; AMPK; HAMPKb; AMPK subunit beta-1; 5'-AMP-activated protein kinase subunit beta-1 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Boil tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified |
| Buffer | PBS (pH 7.4), 0.02% Sodium azide and 50% Glycerol |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | PRKAB1 |
| Gene Full Name | protein kinase, AMP-activated, beta 1 non-catalytic subunit |
| Background | Non-catalytic subunit of AMP-activated protein kinase (AMPK),an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism.In response to reduction of intracellular ATP levels,AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein,carbohydrate and lipid biosynthesis,as well as cell growth and proliferation.AMPK acts via direct phosphorylation of metabolic enzymes,and by longer-term effects via phosphorylation of transcription regulators.Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton;probably by indirectly activating myosin.Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles,via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1,PRKAG2 or PRKAG3). |
| Function | Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3). [UniProt] |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Metabolism antibody; Signaling Transduction antibody |
| Calculated MW | 30 kDa |
| PTM | Phosphorylated when associated with the catalytic subunit (PRKAA1 or PRKAA2). Phosphorylated by ULK1; leading to negatively regulate AMPK activity and suggesting the existence of a regulatory feedback loop between ULK1 and AMPK. |
Images (4) Click the Picture to Zoom In
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ARG54128 anti-AMPK beta 1 antibody ICC/IF image
Immunofluorescence: HeLa cells fixed with 1% Paraformaldehyde and stained with ARG54128 anti-AMPK beta 1 antibody at 1:100 dilution.
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ARG54128 anti-AMPK beta 1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded breast cancer tissue stained with ARG54128 anti-AMPK beta 1 antibody at 1:200 dilution. Antigen Retrieval: Boil tissue section in Citrate buffer (pH 6.0).
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ARG54128 anti-AMPK beta 1 antibody WB image
Western blot: 3T3 cell lysate stained with ARG54128 anti-AMPK beta 1 antibody at 1:1000 dilution.
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ARG54128 anti-AMPK beta 1 antibody IP image
Immunoprecipitation: HeLa cell lysates were immunoprecipitated and stained with ARG54128 anti-AMPK beta 1 antibody.
