ARG43619
anti-AP2 alpha antibody
anti-AP2 alpha antibody for Western blot and Human,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes AP2 alpha |
|---|---|
| Tested Reactivity | Hu, Rat |
| Predict Reactivity | Ms |
| Tested Application | WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | AP2 alpha |
| Antigen Species | Human |
| Immunogen | Recombinant fragment corresponding to a.a. M1-G166 of Human AP2 alpha (TFAP2A). |
| Conjugation | Un-conjugated |
| Protein Full Name | Transcription factor AP-2-alpha |
| Alternate Names | BOFS; TFAP2; Activating enhancer-binding protein 2-alpha; AP2-alpha; Transcription factor AP-2-alpha; AP-2; AP-2alpha; AP-2 transcription factor; Activator protein 2; AP2TF |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| Observed Size | ~45-50 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 5% BSA. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | TFAP2A |
| Gene Full Name | transcription factor AP-2 alpha (activating enhancer binding protein 2 alpha) |
| Background | The protein encoded by this gene is a transcription factor that binds the consensus sequence 5'-GCCNNNGGC-3'. The encoded protein functions as either a homodimer or as a heterodimer with similar family members. This protein activates the transcription of some genes while inhibiting the transcription of others. Defects in this gene are a cause of branchiooculofacial syndrome (BOFS). Three transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Dec 2009] |
| Function | Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. AP-2-alpha is the only AP-2 protein required for early morphogenesis of the lens vesicle. Together with the CITED2 coactivator, stimulates the PITX2 P1 promoter transcription activation. Associates with chromatin to the PITX2 P1 promoter region. [UniProt] |
| Cellular Localization | Nucleus. |
| Calculated MW | 48 kDa |
| PTM | Sumoylated on Lys-10; which inhibits transcriptional activity. |
Images (1) Click the Picture to Zoom In
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ARG43619 anti-AP2 alpha antibody WB image
Western blot: Rat spleen stained with ARG43619 anti-AP2 alpha antibody 1:5000 dilution.
