ARG59798
anti-AP3B1 / HPS2 antibody
anti-AP3B1 / HPS2 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes AP3B1 / HPS2 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | AP3B1 / HPS2 |
| Antigen Species | Human |
| Immunogen | Recombinant fusion protein corresponding to aa. 895-1094 of Human AP3B1 (NP_003655.3). |
| Conjugation | Un-conjugated |
| Alternate Names | Adaptor-related protein complex 3 subunit beta-1; AP-3 complex subunit beta-1; HPS; ADTB3A; Clathrin assembly protein complex 3 beta-1 large chain; Adaptor protein complex AP-3 subunit beta-1; ADTB3; HPS2; PE; Beta-3A-adaptin |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | Mouse liver and SW620 | ||||||
| Observed Size | 132 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | AP3B1 |
| Gene Full Name | adaptor-related protein complex 3, beta 1 subunit |
| Background | This gene encodes a protein that may play a role in organelle biogenesis associated with melanosomes, platelet dense granules, and lysosomes. The encoded protein is part of the heterotetrameric AP-3 protein complex which interacts with the scaffolding protein clathrin. Mutations in this gene are associated with Hermansky-Pudlak syndrome type 2. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2012] |
| Function | Subunit of non-clathrin- and clathrin-associated adaptor protein complex 3 (AP-3) that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules. AP-3 appears to be involved in the sorting of a subset of transmembrane proteins targeted to lysosomes and lysosome-related organelles. In concert with the BLOC-1 complex, AP-3 is required to target cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals. [UniProt] |
| Cellular Localization | Cytoplasmic vesicle, clathrin-coated vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Golgi apparatus. Note=Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex. [UniProt] |
| Calculated MW | 121 kDa |
| PTM | Phosphorylated on serine residues. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG59798 anti-AP3B1 / HPS2 antibody WB image
Western blot: 25 µg of Mouse liver and SW620 cell lysates stained with ARG59798 anti-AP3B1 / HPS2 antibody at 1:1000 dilution.
