ARG57110
anti-ARF1 antibody [1B3]
anti-ARF1 antibody [1B3] for Flow cytometry,ICC/IF,Western blot and Human
Overview
| Product Description | Mouse Monoclonal antibody [1B3] recognizes ARF1 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, ICC/IF, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 1B3 |
| Isotype | IgG1, kappa |
| Target Name | ARF1 |
| Antigen Species | Human |
| Immunogen | Recombinant fragment around aa. 1-181 of Human ARF1 |
| Conjugation | Un-conjugated |
| Alternate Names | ADP-ribosylation factor 1 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 10% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ARF1 |
| Gene Full Name | ADP-ribosylation factor 1 |
| Background | ADP-ribosylation factor 1 (ARF1) is a member of the human ARF gene family. The family members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking as activators of phospholipase D. The gene products, including 6 ARF proteins and 11 ARF-like proteins, constitute a family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2 and ARF3), class II (ARF4 and ARF5) and class III (ARF6), and members of each class share a common gene organization. The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008] |
| Function | GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking among different compartments. Modulates vesicle budding and uncoating within the Golgi complex. Deactivation induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, suggesting a crucial role in protein trafficking. In its GTP-bound form, its triggers the association with coat proteins with the Golgi membrane. The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles. The GTP-bound form interacts with PICK1 to limit PICK1-mediated inhibition of Arp2/3 complex activity; the function is linked to AMPA receptor (AMPAR) trafficking, regulation of synaptic plasicity of excitatory synapses and spine shrinkage during long-term depression (LTD). [UniProt] |
| Calculated MW | 21 kDa |
| PTM | Demyristoylated by S.flexneri cysteine protease IpaJ which cleaves the peptide bond between N-myristoylated Gly-2 and Asn-3. |
Images (4) Click the Picture to Zoom In
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ARG57110 anti-ARF1 antibody [1B3] ICC/IF image
Immunofluorescence: MCF7 cell line stained with ARG57110 anti-ARF1 antibody [1B3] at 1:100 (Green).
DAPI (Blue) for nucleus staining.
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ARG57110 anti-ARF1 antibody [1B3] FACS image
Flow Cytometry: MCF7 cell line stained with ARG57110 anti-ARF1 antibody [1B3] at 2-5 µg for 1x10^6 cells (red line). Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate. Isotype control antibody: Mouse IgG (black line).
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ARG57110 anti-ARF1 antibody [1B3] WB image
Western blot: 40 µg of 1) HeLa, 2) HepG2, 3) Brain tissue lysate, and 4) MCF cell lysates stained with ARG57110 anti-ARF1 antibody [1B3] at 1:1000.
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ARG57110 anti-ARF1 antibody [1B3] ICC/IF image
Immunofluorescence: U87MG cell line stained with ARG57110 anti-ARF1 antibody [1B3] at 1:100 (Green).
DAPI (Blue) for nucleus staining.
