ARG58225

anti-ARF6 antibody

anti-ARF6 antibody for Western blot and Human,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes ARF6
Tested Reactivity Hu, Rat
Tested Application WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name ARF6
Antigen Species Human
Immunogen Synthetic peptide of Human ARF6
Conjugation Un-conjugated
Alternate Names ADP-ribosylation factor 6

Application Instructions

Application Suggestion
Tested Application Dilution
WB1:100 - 1:500
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control A549
Observed Size 19 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 382 Human ARF6

GeneID: 79121 Rat ARF6

Swiss-port # P62330 Human ADP-ribosylation factor 6

Swiss-port # P62332 Rat ADP-ribosylation factor 6

Gene Symbol ARF6
Gene Full Name ADP-ribosylation factor 6
Background This gene encodes a member of the human ARF gene family, which is part of the RAS superfamily. The ARF genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The product of this gene is localized to the plasma membrane, and regulates vesicular trafficking, remodelling of membrane lipids, and signaling pathways that lead to actin remodeling. A pseudogene of this gene is located on chromosome 7. [provided by RefSeq, Jul 2008]
Function GTP-binding protein involved in protein trafficking that regulates endocytic recycling and cytoskeleton remodeling. Required for normal completion of mitotic cytokinesis. Plays a role in the reorganization of the actin cytoskeleton and the formation of stress fibers. May also modulate vesicle budding and uncoating within the Golgi apparatus. Involved in the regulation of dendritic spine development, contributing to the regulation of dendritic branching and filopodia extension. Functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. [UniProt]
Cellular Localization Cell membrane, Cell projection, Cleavage furrow, Cytoplasm, Endosome membrane, Golgi apparatus, Lipid-anchor, Midbody, Recycling endosome membrane, filopodium membrane. [UniProt]
Calculated MW 20 kDa

Images (1) Click the Picture to Zoom In

  • ARG58225 anti-ARF6 antibody WB image

    Western blot: 25 µg of A549 cell lysate stained with ARG58225 anti-ARF6 antibody at 1:250 dilution.