ARG63254
anti-ARIH1 / HHARI antibody
anti-ARIH1 / HHARI antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Cell Biology and Cellular Response antibody; Metabolism antibody; Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes ARIH1 / HHARI |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Cow |
| Tested Application | IHC-P, WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | ARIH1 / HHARI |
| Antigen Species | Human |
| Immunogen | C-HEGYEKDLWEYIED |
| Conjugation | Un-conjugated |
| Alternate Names | UbcH7-binding protein; HARI; EC 6.3.2.-; E3 ubiquitin-protein ligase ARIH1; ARI-1; Ubiquitin-conjugating enzyme E2-binding protein 1; Protein ariadne-1 homolog; MOP-6; H7-AP2; UbcM4-interacting protein; HHARI; ARI; Monocyte protein 6; UBCH7BP |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by antigen affinity chromatography. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ARIH1 |
| Gene Full Name | ariadne RBR E3 ubiquitin protein ligase 1 |
| Function | E3 ubiquitin-protein ligase, which catalyzes polyubiquitination of target proteins together with ubiquitin-conjugating enzyme E2 UBE2L3. May play a role in protein translation by mediating polyubiquitination of EIF4E2, leading to its subsequent degradation. Acts as the ligase involved in ISGylation of EIF4E2. [UniProt] |
| Research Area | Cell Biology and Cellular Response antibody; Metabolism antibody; Signaling Transduction antibody |
| Calculated MW | 64 kDa |
Images (2) Click the Picture to Zoom In
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ARG63254 anti-ARIH1 / HHARI antibody WB image
Western Blot: U937 lysate (RIPA buffer, 30µg total protein per lane) stained with ARG63254 anti-ARIH1 / HHARI antibody at 0.5 µg/ml dilution.
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ARG63254 anti-ARIH1 / HHARI antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human adrenal gland tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63254 anti-ARIH1 / HHARI antibody at 5 µg/ml dilution followed by AP-staining.
