ARG64833
anti-ARK5 / NUAK1 antibody
anti-ARK5 / NUAK1 antibody for Flow cytometry,ICC/IF and Human
Cancer antibody; Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes ARK5 / NUAK1 |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Cow, Rat |
| Tested Application | FACS, ICC/IF |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | ARK5 / NUAK1 |
| Antigen Species | Human |
| Immunogen | C-QNRPRPQYLKRYRNR |
| Conjugation | Un-conjugated |
| Alternate Names | ARK5; NUAK family SNF1-like kinase 1; AMPK-related protein kinase 5; Omphalocele kinase 1; EC 2.7.11.1 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by antigen affinity chromatography. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | NUAK1 |
| Gene Full Name | NUAK family, SNF1-like kinase, 1 |
| Function | Serine/threonine-protein kinase involved in various processes such as cell adhesion, regulation of cell ploidy and senescence, cell proliferation and tumor progression. Phosphorylates ATM, CASP6, LATS1, PPP1R12A and p53/TP53. Acts as a regulator of cellular senescence and cellular ploidy by mediating phosphorylation of 'Ser-464' of LATS1, thereby controlling its stability. Controls cell adhesion by regulating activity of the myosin protein phosphatase 1 (PP1) complex. Acts by mediating phosphorylation of PPP1R12A subunit of myosin PP1: phosphorylated PPP1R12A then interacts with 14-3-3, leading to reduced dephosphorylation of myosin MLC2 by myosin PP1. May be involved in DNA damage response: phosphorylates p53/TP53 at 'Ser-15' and 'Ser-392' and is recruited to the CDKN1A/WAF1 promoter to participate to transcription activation by p53/TP53. May also act as a tumor malignancy-associated factor by promoting tumor invasion and metastasis under regulation and phosphorylation by AKT1. Suppresses Fas-induced apoptosis by mediating phosphorylation of CASP6, thereby suppressing the activation of the caspase and the subsequent cleavage of CFLAR. Regulates UV radiation-induced DNA damage response mediated by CDKN1A. In association with STK11, phosphorylates CDKN1A in response to UV radiation and contributes to its degradation which is necessary for optimal DNA repair (PubMed:25329316). [UniProt] |
| Research Area | Cancer antibody; Signaling Transduction antibody |
| Calculated MW | 74 kDa |
| PTM | Ubiquitinated with 'Lys-29'- and 'Lys-33'-linked polyubiquitins which appear to impede LKB1-mediated phosphorylation. Deubiquitinated by USP9X. Phosphorylated at Thr-211 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39. Not dephosphorylated by the myosin PP1 complex when regulating its activity, due to the presence of PPP1R12A, which prevents myosin PP1 from dephosphorylating NUAK1. Phosphorylated by STK38L upon stimulation with IGF1. |
Images (2) Click the Picture to Zoom In
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ARG64833 anti-ARK5 / NUAK1 antibody ICC/IF image
Immunofluorescence: Paraformaldehyde fixed U2OS cells permeabilized with 0.15% Triton. Cells were stained with ARG64833 anti-ARK5 / NUAK1 antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.
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ARG64833 anti-ARK5 / NUAK1 antibody FACS image
Flow Cytometry: Paraformaldehyde-fixed A431 cells permeabilized with 0.5% Triton. Cells were stained with ARG64833 anti-ARK5 / NUAK1 antibody (blue line) at 10 µg/ml dilution for 1 hour, followed by incubation with Alexa FluorR 488 labelled secondary antibody. IgG control: Unimmunized goat IgG (black line).
