Overview

Product Description	Rabbit Polyclonal antibody recognizes ASAH2
Tested Reactivity	Hu, Ms, Rat
Tested Application	WB
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Target Name	ASAH2
Antigen Species	Human
Immunogen	Recombinant Protein of Human ASAH2.
Conjugation	Un-conjugated
Alternate Names	hCD; EC 3.5.1.23; HNAC1; LCDase; Acylsphingosine deacylase 2; Neutral ceramidase; NCDase; Non-lysosomal ceramidase; BCDase; N-CDase; N-acylsphingosine amidohydrolase 2

Application Instructions

Application Suggestion	Tested Application Dilution WB 1:500 - 2000
Application Note	* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control	Mouse kidney

Properties

Form	Liquid
Purification	Affinity purification with immunogen.
Buffer	PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative	0.02% Sodium azide
Stabilizer	50% Glycerol
Storage Instruction	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links	GeneID: 114104 Rat ASAH2 GeneID: 54447 Mouse ASAH2 GeneID: 56624 Human ASAH2 Swiss-port # Q91XT9 Rat Neutral ceramidase Swiss-port # Q9JHE3 Mouse Neutral ceramidase Swiss-port # Q9NR71 Human Neutral ceramidase More
Gene Symbol	ASAH2
Gene Full Name	N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2
Background	Ceramidases (EC 3.5.1.23), such as ASAH2, catalyze hydrolysis of the N-acyl linkage of ceramide, a second messenger in a variety of cellular events, to produce sphingosine. Sphingosine exerts both mitogenic and apoptosis-inducing activities, and its phosphorylated form functions as an intra- and intercellular second messenger (see MIM 603730) (Mitsutake et al., 2001 [PubMed 11328816]).[supplied by OMIM, Mar 2008]
Function	Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid at an optimal pH of 6.5-8.5. Acts as a key regulator of sphingolipid signaling metabolites by generating sphingosine at the cell surface. Acts as a repressor of apoptosis both by reducing C16-ceramide, thereby preventing ceramide-induced apoptosis, and generating sphingosine, a precursor of the antiapoptotic factor sphingosine 1-phosphate. Probably involved in the digestion of dietary sphingolipids in intestine by acting as a key enzyme for the catabolism of dietary sphingolipids and regulating the levels of bioactive sphingolipid metabolites in the intestinal tract. [UniProt]
Calculated MW	86 kDa
PTM	N-glycosylated. Required for enzyme activity (By similarity). O-glycosylated. Required to retain it as a type II membrane protein at the cell surface. Phosphorylated. May prevent ubiquitination and subsequent degradation (By similarity). Ubiquitinated, leading to its degradation by the proteasome. Ubiquitination is triggered by nitric oxid (By similarity).

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