ARG42092
anti-ASCL3 antibody
anti-ASCL3 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Overview
| Product Description | Goat Polyclonal antibody recognizes ASCL3 |
|---|---|
| Tested Reactivity | Hu, Ms |
| Predict Reactivity | Cow |
| Tested Application | IHC-P, WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | ASCL3 |
| Antigen Species | Mouse |
| Immunogen | Synthetic peptide around the internal region of Mouse Ascl3. (C-HLPEDYLEKRLSK) (NP_064435.1) |
| Conjugation | Un-conjugated |
| Alternate Names | HASH3; SGN1; Achaete-scute homolog 3; bHLH transcriptional regulator Sgn-1; hASH3; ASH-3; bHLHa42; Class A basic helix-loop-helix protein 42 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Positive Control | Mouse liver | ||||||
| Observed Size | ~ 22 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ASCL3 |
| Gene Full Name | achaete-scute family bHLH transcription factor 3 |
| Background | Basic helix-loop-helix transcription factors, such as ASCL3, are essential for the determination of cell fate and the development and differentiation of numerous tissues (Jonsson et al., 2004 [PubMed 15475265]).[supplied by OMIM, Mar 2008] |
| Function | Transcriptional repressor. Inhibits myogenesis (By similarity). [UniProt] |
| Cellular Localization | Nucleus. [UniProt] |
| Calculated MW | 20 kDa |
Images (2) Click the Picture to Zoom In
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ARG42092 anti-ASCL3 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human cortex tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG42092 anti-ASCL3 antibody at 3.75 µg/ml dilution.
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ARG42092 anti-ASCL3 antibody WB image
Western blot: 35 µg of Mouse liver lysate (in RIPA buffer) stained with ARG42092 anti-ASCL3 antibody at 0.1 µg/ml dilution and incubated at RT for 1 hour.
