Overview

Product Description	Rabbit Polyclonal antibody recognizes ATP1B3
Tested Reactivity	Hu, Ms, Rat
Tested Application	IHC-P, WB
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Target Name	ATP1B3
Antigen Species	Human
Immunogen	Synthetic peptide derived from Human ATP1B3.
Conjugation	Un-conjugated
Alternate Names	Sodium/potassium-dependent ATPase subunit beta-3; Sodium/potassium-transporting ATPase subunit beta-3; ATPB-3; CD antigen CD298; CD298

Application Instructions

Application Suggestion	Tested Application Dilution IHC-P 1:50 - 1:200 WB 1:500 - 1:2000
Application Note	* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form	Liquid
Purification	Affinity purified.
Buffer	PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative	0.02% Sodium azide
Stabilizer	50% Glycerol
Storage Instruction	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links	GeneID: 11933 Mouse ATP1B3 GeneID: 25390 Rat ATP1B3 GeneID: 483 Human ATP1B3 Swiss-port # P54709 Human Sodium/potassium-transporting ATPase subunit beta-3 Swiss-port # P97370 Mouse Sodium/potassium-transporting ATPase subunit beta-3 Swiss-port # Q63377 Rat Sodium/potassium-transporting ATPase subunit beta-3 More
Gene Symbol	ATP1B3
Gene Full Name	ATPase, Na+/K+ transporting, beta 3 polypeptide
Background	The protein encoded by this gene belongs to the family of Na+/K+ and H+/K+ ATPases beta chain proteins, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. The glycoprotein subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes a beta 3 subunit. This gene encodes a beta 3 subunit. A pseudogene exists for this gene, and it is located on chromosome 2. [provided by RefSeq, Jul 2008]
Function	This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The exact function of the beta-3 subunit is not known. [UniProt]
Cellular Localization	Cell membrane; Single-pass type II membrane protein. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. [UniProt]
Calculated MW	32 kDa

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