ARG40089
anti-ATP6V1G3 antibody
anti-ATP6V1G3 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Mouse
Overview
| Product Description | Rabbit Polyclonal antibody recognizes ATP6V1G3 |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | ATP6V1G3 |
| Antigen Species | Human |
| Immunogen | Recombinant fusion protein corresponding to aa. 1-118 of Human ATP6V1G3 (NP_573569.1). |
| Conjugation | Un-conjugated |
| Alternate Names | ATP6G3; Vacuolar proton pump subunit G 3; Vma10; V-ATPase subunit G 3; V-type proton ATPase subunit G 3; V-ATPase 13 kDa subunit 3 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | Mouse kidney | ||||||
| Observed Size | 14 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ATP6V1G3 |
| Gene Full Name | ATPase, H+ transporting, lysosomal 13kDa, V1 subunit G3 |
| Background | This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c'' and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This gene encodes one of three G subunit proteins. Transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Function | Catalytic subunit of the peripheral V1 complex of vacuolar ATPase (V-ATPase). V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. [UniProt] |
| Calculated MW | 14 kDa |
Images (2) Click the Picture to Zoom In
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ARG40089 anti-ATP6V1G3 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse kidney stained with ARG40089 anti-ATP6V1G3 antibody at 1:100 dilution.
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ARG40089 anti-ATP6V1G3 antibody WB image
Western blot: 25 µg of Mouse kidney lysate stained with ARG40089 anti-ATP6V1G3 antibody at 1:1000 dilution.
