ARG10693
anti-Aldolase antibody [E9]
anti-Aldolase antibody [E9] for ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat,Cow,Horse,Pig
Publication1
Overview
| Product Description | Mouse Monoclonal antibody [E9] recognizes Aldolase |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Cow, Hrs, Pig |
| Predict Reactivity | Chk |
| Tested Application | ICC/IF, IHC-Fr, IHC-P, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | E9 |
| Isotype | IgG1 |
| Target Name | Aldolase |
| Antigen Species | Human |
| Immunogen | Recombinant Human full-length Aldolase C. |
| Conjugation | Un-conjugated |
| Alternate Names | Lung cancer antigen NY-LU-1; HEL-S-87p; GSD12; Fructose-bisphosphate aldolase A; Muscle-type aldolase; EC 4.1.2.13; ALDA |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification. |
| Buffer | PBS and 50% Glycerol. |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ALDOA |
| Gene Full Name | aldolase A, fructose-bisphosphate |
| Background | The protein encoded by this gene, Aldolase A (fructose-bisphosphate aldolase), is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Three aldolase isozymes (A, B, and C), encoded by three different genes, are differentially expressed during development. Aldolase A is found in the developing embryo and is produced in even greater amounts in adult muscle. Aldolase A expression is repressed in adult liver, kidney and intestine and similar to aldolase C levels in brain and other nervous tissue. Aldolase A deficiency has been associated with myopathy and hemolytic anemia. Alternative splicing and alternative promoter usage results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 3 and 10. [provided by RefSeq, Aug 2011] |
| Function | Plays a key role in glycolysis and gluconeogenesis. In addition, may also function as scaffolding protein (By similarity). [UniProt] |
| Calculated MW | 39 kDa |
Images (4) Click the Picture to Zoom In
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ARG10693 anti-Aldolase antibody [E9] IHC-P image
Immunohistochemistry: Rat Right ventricular stained with ARG10693 anti-Aldolase antibody [E9] at 1:1000 dilution.
From Zhang Mingjing et al. J Cell Mol Med. (2018), doi: 10.1111/jcmm.14000, Fig. 6A.
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ARG10693 anti-Aldolase antibody [E9] ICC/IF image
Immunocytochemistry: Rat mixed neuron / glial cultures stained with ARG10693 anti-Aldolase antibody [E9] (red) and co-stained with chicken antibody to GFAP (green). ARG10693 anti-Aldolase antibody [E9] antibody reveals strong cytoplasmic staining in astrocytes. Blue is a DNA stain. Aldolase stains the astrocytes cell body and processes, whereas GFAP labels the intermediate filament of the cytoskeleton in subset of astrocytes.
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ARG10693 anti-Aldolase antibody [E9] WB image
Western blot: Rat brain lysate stained with ARG10693 anti-Aldolase antibody [E9]. This antibody binds to an epitope shared by the closely related Aldolase A, B and C, recognizing all three on WB.
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ARG10693 anti-Aldolase antibody [E9] WB image
Western blot: Recombinant Human Aldolase A, B and C with three monoclonal antibodies as indicated showing binding to all three gene products. Lane labelled S show molecular weight standards, while lanes A, B and C contain recombinant full length His-tagged Human Aldolase A, B and C respectively. The epitope for ARG10693 anti-Aldolase antibody [E9] is within the core of the three Aldolase molecules which are less conserved than the N- and C-termini where our Aldolase C specific antibodies bind. Clone 4A9 binds to the N-terminal peptide and clone 1A1 binds to the C-terminal peptide.
Customer's Feedback
Excellent
Review for anti-Aldolase antibody [E9]
Application：WB
Sample：293T
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Excellent
Review for anti-Aldolase antibody [E9]
Application：WB
Sample：HeLa
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Specific References
Bilateral sympathetic stellate ganglionectomy attenuates myocardial remodelling and fibrosis in a rat model of chronic volume overload
IHC-P / Rat
