ARG10554
anti-B Raf antibody [G11-G]
anti-B Raf antibody [G11-G] for IHC-Formalin-fixed paraffin-embedded sections and Human,Mouse,Rat
Publication1
Overview
| Product Description | Rabbit Monoclonal antibody [G11-G] recognizes B Raf |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | IHC-P |
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | G11-G |
| Isotype | IgG |
| Target Name | B Raf |
| Antigen Species | Human |
| Immunogen | Synthetic peptide around Val600 of Human B-raf. (corresponds to Val637 of Mouse or Rat B-raf). |
| Conjugation | Un-conjugated |
| Alternate Names | BRAF1; v-Raf murine sarcoma viral oncogene homolog B1; Proto-oncogene B-Raf; p94; Serine/threonine-protein kinase B-raf; RAFB1; EC 2.7.11.1; B-RAF1; NS7 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Buffer | 20 mM Tris-HCl (pH 8.0), 0.05% Sodium azide and 20 mg/ml BSA |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 20 mg/ml BSA |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P15056 Human Serine/threonine-protein kinase B-raf Swiss-port # P28028 Mouse Serine/threonine-protein kinase B-raf |
|---|---|
| Gene Symbol | BRAF |
| Gene Full Name | B-Raf proto-oncogene, serine/threonine kinase |
| Background | This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene. [provided by RefSeq, Jul 2008] |
| Function | Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway. [UniProt] |
| Calculated MW | 84 kDa |
| PTM | Phosphorylation at Ser-365 by SGK1 inhibits its activity. Methylation at Arg-671 decreases stability and kinase activity. Ubiquitinated by RNF149; which leads to proteasomal degradation. Polyubiquitinated at Lys-578 in response to EGF. |
Images (2) Click the Picture to Zoom In
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ARG10554 anti-B Raf antibody [G11-G] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human epithelium and stromal cells in normal bowel mucosa tissue (4 µm sections) stained with ARG10554 anti-B Raf antibody [G11-G].
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ARG10554 anti-B Raf antibody [G11-G] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human nuclei of basal cell carcinoma of the skin and dermal fibroblasts tissue (4 µm sections) stained with ARG10554 anti-B Raf antibody [G11-G].
Specific References
BRAF V600E mutant colorectal cancer cells mediate local immunosuppressive microenvironment through exosomal long noncoding RNAs
WB / Human
