ARG64303
anti-B7-H4 antibody
anti-B7-H4 antibody for Flow cytometry,ICC/IF and Human
Cancer antibody; Cell Biology and Cellular Response antibody; Immune System antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes B7-H4 |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat, Dog |
| Tested Application | FACS, ICC/IF |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | B7-H4 |
| Antigen Species | Human |
| Immunogen | C-SKGKGNANLEYK |
| Conjugation | Un-conjugated |
| Alternate Names | B7H4; B7 homolog 4; Protein B7S1; V-set domain-containing T-cell activation inhibitor 1; B7-H4; B7X; T-cell costimulatory molecule B7x; B7h.5; VCTN1; PRO1291; Immune costimulatory protein B7-H4; B7S1 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by antigen affinity chromatography. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q7Z7D3 Human V-set domain-containing T-cell activation inhibitor 1 |
|---|---|
| Background | This gene encodes a protein belonging to the B7 costimulatory protein family. Proteins in this family are present on the surface of antigen-presenting cells and interact with ligand bound to receptors on the surface of T cells. Studies have shown that high levels of the encoded protein has been correlated with tumor progression. A pseudogene of this gene is located on chromosome 20. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011] |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Immune System antibody |
| Calculated MW | 31 kDa |
| PTM | N-glycosylated. |
Images (2) Click the Picture to Zoom In
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ARG64303 anti-B7-H4 antibody ICC/IF image
Immunofluorescence: Paraformaldehyde fixed MCF7 cells permeabilized with 0.15% Triton. Cells were stained with ARG64303 anti-B7-H4 antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.
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ARG64303 anti-B7-H4 antibody FACS image
Flow Cytometry: Paraformaldehyde-fixed MCF7 cells permeabilized with 0.5% Triton. Cells were stained with ARG64303 anti-B7-H4 antibody (blue line) at 10 µg/ml dilution for 1 hour, followed by incubation with Alexa FluorR 488 labelled secondary antibody. IgG control: Unimmunized goat IgG (black line).
