ARG54377
anti-BACE2 antibody
anti-BACE2 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Cell Biology and Cellular Response antibody; Neuroscience antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes BACE2 |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | IHC-P, WB |
| Specificity | This antibody recognizes human, mouse, and rat BACE2 (approx. 75 kD). |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | BACE2 |
| Antigen Species | Human |
| Immunogen | Peptide corresponding to aa 44-59 of human BACE2. |
| Conjugation | Un-conjugated |
| Alternate Names | Beta-site APP cleaving enzyme 2; ASP21; EC 3.4.23.45; Membrane-associated aspartic protease 1; DRAP; BAE2; Down region aspartic protease; CEAP1; Beta-secretase 2; Memapsin-1; Aspartyl protease 1; Aspartic-like protease 56 kDa; ALP56; AEPLC; Asp 1; Theta-secretase; CDA13; Beta-site amyloid precursor protein cleaving enzyme 2; ASP1 |
Application Instructions
| Application Suggestion |
|
||||||
|---|---|---|---|---|---|---|---|
| Application Note | Western blot: use at 1:500 - 1:1,000 dilution. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
||||||
| Positive Control | Human heart tissue lysate |
Properties
| Form | Liquid |
|---|---|
| Purification | Immunoaffinity chroma-tography |
| Buffer | PBS (pH 7.4) and 0.02% Sodium azide |
| Preservative | 0.02% Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | BACE2 |
| Gene Full Name | beta-site APP-cleaving enzyme 2 |
| Background | Accumulation of the amyloid-β peptide (Aβ)in the cerebral cortex is a critical event in the pathogenesis of Alzheimer's disease. Aβ peptide is generated as a result of proteolytic cleavage of the β-amyloid protein precursor (APP) at β- and γ-sites. β-secretase was recently identified and designated beta-site APP cleaving enzyme (BACE) and aspartyl protease 2 (Asp2). A BACE homolog was recently identified and designated BACE2, or Asp1, DRAP (for Down region aspartic protease), or memapsin 1. BACE2 cleaves APP at the β-site and at a different site within Aβ.BACE2 is found on chromosome 21q22 3, the 'Down critical region', suggesting that BACE2 and Aβ may also contribute to the pathogenesis of Down syndrome. |
| Function | Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves APP, between residues 690 and 691, leading to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase. It has also been shown that it can cleave APP between residues 671 and 672. [UniProt] |
| Research Area | Cell Biology and Cellular Response antibody; Neuroscience antibody |
| Calculated MW | 56 kDa |
| PTM | Undergoes autoproteolytic cleavage. Glycosylated. |
Images (2) Click the Picture to Zoom In
-
ARG54377 anti-BACE2 antibody IHC validated image
Immunohistochemistry: Mouse heart stained with ARG54377 anti-BACE2 antibody at 10 μg/ml dilution.
-
ARG54377 anti-BACE2 antibody WB validated image
Western blot: Human heart stained with ARG54377 anti-BACE2 antibody at 1 μg/ml dilution.
