ARG63355
anti-BAP / SIL1 antibody
anti-BAP / SIL1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes BAP / SIL1 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IHC-P, WB |
| Specificity | Variants (NP_071909.1; NP_001032722.1) encode the same isoform. |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | BAP / SIL1 |
| Antigen Species | Human |
| Immunogen | C-ELLGSVNSLLKELR |
| Conjugation | Un-conjugated |
| Alternate Names | Nucleotide exchange factor SIL1; BiP-associated protein; ULG5; MSS; BAP |
Application Instructions
| Application Suggestion |
|
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|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by antigen affinity chromatography. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | This gene encodes a resident endoplasmic reticulum (ER), N-linked glycoprotein with an N-terminal ER targeting sequence, 2 putative N-glycosylation sites, and a C-terminal ER retention signal. This protein functions as a nucleotide exchange factor for another unfolded protein response protein. Mutations in this gene have been associated with Marinesco-Sjogren syndrome. Alternate transcriptional splice variants have been characterized. [provided by RefSeq, Jul 2008] |
| Research Area | Signaling Transduction antibody |
| Calculated MW | 52 kDa |
| PTM | N-glycosylated. |
Images (3) Click the Picture to Zoom In
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ARG63355 anti-BAP / SIL1 antibody WB image
Western Blot: Human Kidney lysate (RIPA buffer, 30µg total protein per lane) stained with ARG63355 anti-BAP / SIL1 antibody at 1 µg/ml dilution.
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ARG63355 anti-BAP / SIL1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human liver tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63355 anti-BAP / SIL1 antibody at 5 µg/ml dilution followed by AP-staining.
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ARG63355 anti-BAP / SIL1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human testis tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63355 anti-BAP / SIL1 antibody at 5 µg/ml dilution followed by AP-staining.
