ARG52561
anti-Bcl 6 antibody [SP155]
anti-Bcl 6 antibody [SP155] for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections and Human
Cancer antibody; Gene Regulation antibody
Overview
| Product Description | Rabbit Monoclonal antibody [SP155] recognizes Bcl 6 |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Rat |
| Tested Application | FACS, IHC-P |
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | SP155 |
| Isotype | IgG |
| Target Name | Bcl 6 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide derived from the C-terminus of human BCL-6 protein. |
| Conjugation | Un-conjugated |
| Alternate Names | ZNF51; LAZ3; Zinc finger protein 51; Protein LAZ-3; Zinc finger and BTB domain-containing protein 27; B-cell lymphoma 6 protein; B-cell lymphoma 5 protein; BCL5; BCL6A; BCL-5; ZBTB27; BCL-6 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. Incubation Time: 10 min at RT. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Positive Control | Tonsil |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified by protein A/G |
| Buffer | PBS (pH 7.6), 1% BSA and < 0.1% Sodium azide |
| Preservative | < 0.1% Sodium azide |
| Stabilizer | 1% BSA |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | B cell lymphoma 6 protein (Bcl-6) proto-oncogene product (a Kruppel-type zinc-finger protein) is mainly expressed in normal germinal center B cells and related lymphomas. Bcl-6 is involved in chromosome rearrangements at 3q27 in non-Hodgkin’s lymphomas and Bcl-6 rearrangements have been detected in 33%-45% of diffuse large B cell lymphomas. Bcl-6 has been detected immunohistochemically in follicular lymphomas, diffuse large B cell lymphomas, Burkitt lymphomas and in nodular, lymphocyte predominant Hodgkin’s disease. |
| Cellular Localization | Nucleus |
| Highlight | Related products: BCL6 antibodies; Anti-Rabbit IgG secondary antibodies; Related news: Lymphoma |
| Research Area | Cancer antibody; Gene Regulation antibody |
| Calculated MW | 79 kDa |
| PTM | Phosphorylated by MAPK1 in response to antigen receptor activation at Ser-333 and Ser-343. Phosphorylated by ATM in response to genotoxic stress. Phosphorylation induces its degradation by ubiquitin/proteasome pathway. Polyubiquitinated. Polyubiquitinated by SCF(FBXO11), leading to its degradation by the proteasome. Acetylated at Lys-379 by EP300 which inhibits the interaction with NuRD complex and the transcriptional repressor function. Deacetylated by HDAC- and SIR2-dependent pathways. |
Images (7) Click the Picture to Zoom In
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ARG52561 anti-Bcl 6 antibody [SP155] IHC-P image
Immunohistochemistry: Human Tonsil stained with ARG52561 anti-Bcl 6 antibody [SP155].
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ARG52561 anti-Bcl 6 antibody [SP155] IHC-P image
Immunohistochemistry: Human B-Cell Lymphoma stained with ARG52561 anti-Bcl 6 antibody [SP155].
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ARG52561 anti-Bcl 6 antibody [SP155] IHC-P image
Immunohistochemistry: Human Tonsil stained with ARG52561 anti-Bcl 6 antibody [SP155].
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ARG52561 anti-Bcl 6 antibody [SP155] IHC-P image
Immunohistochemistry: Human B-Cell Lymphoma stained with ARG52561 anti-Bcl 6 antibody [SP155].
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ARG52561 anti-Bcl 6 antibody [SP155] IHC-P image
Immunohistochemistry: Human HK Lymphoma stained with ARG52561 anti-Bcl 6 antibody [SP155].
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ARG52561 anti-Bcl 6 antibody [SP155] IHC-P image
Immunohistochemistry: Human Follicular Lymphoma stained with ARG52561 anti-Bcl 6 antibody [SP155].
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ARG52561 anti-Bcl 6 antibody [SP155] IHC-P image
Immunohistochemistry: Human Diffuse B-Cell Lymphoma stained with ARG52561 anti-Bcl 6 antibody [SP155].
