ARG63145
anti-Bcl 7A antibody
anti-Bcl 7A antibody for Flow cytometry,ICC/IF,Western blot and Human
Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes Bcl 7A |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat, Cow, Dog, Pig |
| Tested Application | FACS, ICC/IF, WB |
| Specificity | This antibody is expected to recognize both reported isoforms (NP_066273.1; NP_001019979.1). |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Bcl 7A |
| Antigen Species | Human |
| Immunogen | C-MKLEASQQNSEEM |
| Conjugation | Un-conjugated |
| Alternate Names | B-cell CLL/lymphoma 7 protein family member A; BCL7 |
Application Instructions
| Application Suggestion |
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| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by antigen affinity chromatography. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q4VC05 Human B-cell CLL/lymphoma 7 protein family member A |
|---|---|
| Background | This gene is directly involved, with Myc and IgH, in a three-way gene translocation in a Burkitt lymphoma cell line. As a result of the gene translocation, the N-terminal region of the gene product is disrupted, which is thought to be related to the pathogenesis of a subset of high-grade B cell non-Hodgkin lymphoma. The N-terminal segment involved in the translocation includes the region that shares a strong sequence similarity with those of BCL7B and BCL7C. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody |
| Calculated MW | 23 kDa |
Images (3) Click the Picture to Zoom In
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ARG63145 anti-Bcl 7A antibody WB image
Western blot: Human Lymphnode lysate (RIPA buffer, 35 µg total protein per lane) stained with ARG63145 anti-Bcl 7A antibody at 1 µg/ml dilution.
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ARG63145 anti-Bcl 7A antibody ICC/IF image
Immunofluorescence: Paraformaldehyde fixed U2OS cells permeabilized with 0.15% Triton. Cells were stained with ARG63145 anti-Bcl 7A antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Phalloidin (red) for Actin filaments staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.
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ARG63145 anti-Bcl 7A antibody FACS image
Flow Cytometry: Paraformaldehyde-fixed Daudi cells permeabilized with 0.5% Triton. Cells were stained with ARG63145 anti-Bcl 7A antibody (blue line) at 10 µg/ml dilution for 1 hour, followed by incubation with Alexa FluorR 488 labelled secondary antibody. IgG control: Unimmunized goat IgG (black line).
