ARG45539

anti-Borealin / CDCA8 antibody

anti-Borealin / CDCA8 antibody for Flow cytometry,ICC/IF,Western blot and Human

Overview

Product Description Rabbit Polyclonal antibody recognizes Borealin / CDCA8
Tested Reactivity Hu
Tested Application FACS, ICC/IF, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Borealin / CDCA8
Antigen Species Human
Immunogen Recombinant protein containing to human Borealin / CDCA8.
Conjugation Un-conjugated
Alternate Names CDCA8; Cell Division Cycle Associated 8; Borealin; MESRGP; DasraB; BOR; Pluripotent Embryonic Stem Cell-Related Gene 3 Protein; Cell Division Cycle-Associated Protein 8; FLJ12042; HDasra-B; Dasra-B; Dasra B; PESCRG3

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 - 3 µg/10^6 cells
ICC/IF5 μg/ml
WB0.25-0.5 μg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size 35 kDa

Properties

Form Liquid
Purification Affinity purified
Buffer 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose.
Stabilizer 4% Trehalose
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 55143 Human CDCA8

Swiss-port # Q53HL2 Human Borealin

Gene Symbol CDCA8
Gene Full Name Cell Division Cycle Associated 8
Background This gene encodes a component of the chromosomal passenger complex. This complex is an essential regulator of mitosis and cell division. This protein is cell-cycle regulated and is required for chromatin-induced microtubule stabilization and spindle formation. Alternate splicing results in multiple transcript variants. Pseudgenes of this gene are found on chromosomes 7, 8 and 16. [provided by RefSeq, Apr 2013]
Function Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Major effector of the TTK kinase in the control of attachment-error-correction and chromosome alignment. [UniProt]
Cellular Localization Centromere; Chromosome; Cytoplasm; Cytoskeleton; Nucleus. [UniProt]
Calculated MW 31 kDa
PTM Isopeptide bond; Phosphoprotein; Ubl conjugation. [UniProt]