ARG22738
anti-Bu1A antibody [L22] (FITC)
anti-Bu1A antibody [L22] (FITC) for Flow cytometry and Chicken
Overview
| Product Description | FITC-conjugated Mouse Monoclonal antibody [L22] recognizes Bu1A This antibody recognizes 'a' allele of the polymorphic cell surface antigen Bu-1, also known as chB6. Bu-1a is a 70 kDa alloantigen expressed on B cells and a subset of macrophages. Reports suggest that Bu-1 is expressed on B cell precursors at early stages of development (Houssaint et al. 1991). Weak cross-reactivity with Bu-1b may be seen at higher antibody levels. This may be removed by appropriate titration. Mouse anti Chicken BU-1a, clone L22 cross reacts with quail Bu-1 but does not appear reactive with either turkey or guinea fowl tissues (Igyarto et al. 2008). |
|---|---|
| Tested Reactivity | Chk |
| Tested Application | FACS |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | L22 |
| Isotype | IgG1 |
| Target Name | Bu1A |
| Antigen Species | Chicken |
| Immunogen | Chicken Bursa cells. |
| Conjugation | FITC |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS, 0.09% Sodium azide and 1% BSA. |
| Preservative | 0.09% Sodium azide |
| Stabilizer | 1% BSA |
| Concentration | 0.1 mg/ml |
| Storage Instruction | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
