ARG58342
anti-CARS / CysRS antibody
anti-CARS / CysRS antibody for ICC/IF,Western blot and Human,Mouse
Overview
| Product Description | Rabbit Polyclonal antibody recognizes CARS / CysRS |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | CARS / CysRS |
| Antigen Species | Human |
| Immunogen | Recombinant fusion protein corresponding to aa. 469-748 of Human CARS (NP_001742.1). |
| Conjugation | Un-conjugated |
| Alternate Names | Cysteine--tRNA ligase, cytoplasmic; Cysteinyl-tRNA synthetase; CYSRS; MGC:11246; EC 6.1.1.16; CARS1; CysRS |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | HeLa | ||||||
| Observed Size | 85 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P49589 Human Cysteine--tRNA ligase, cytoplasmic Swiss-port # Q9ER72 Mouse Cysteine--tRNA ligase, cytoplasmic |
|---|---|
| Gene Symbol | CARS |
| Gene Full Name | cysteinyl-tRNA synthetase |
| Background | This gene encodes a class 1 aminoacyl-tRNA synthetase, cysteinyl-tRNA synthetase. Each of the twenty aminoacyl-tRNA synthetases catalyzes the aminoacylation of a specific tRNA or tRNA isoaccepting family with the cognate amino acid. This gene is one of several located near the imprinted gene domain on chromosome 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian and breast cancers. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Aug 2010] |
| Cellular Localization | Cytoplasm. [UniProt] |
| Calculated MW | 85 kDa |
Images (2) Click the Picture to Zoom In
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ARG58342 anti-CARS / CysRS antibody ICC/IF image
Immunofluorescence: HeLa cells stained with ARG58342 anti-CARS / CysRS antibody.
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ARG58342 anti-CARS / CysRS antibody WB image
Western blot: 25 µg of HeLa cell lysate stained with ARG58342 anti-CARS / CysRS antibody at 1:1000 dilution.
