Overview

Product Description	Rabbit Polyclonal antibody recognizes CBLB
Tested Reactivity	Hu, Ms, Rat
Tested Application	WB
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Target Name	CBLB
Antigen Species	Human
Immunogen	Recombinant Protein of Human CBLB.
Conjugation	Un-conjugated
Alternate Names	Nbla00127; EC 6.3.2.-; RNF56; Signal transduction protein CBL-B; Casitas B-lineage lymphoma proto-oncogene b; SH3-binding protein CBL-B; Cbl-b; E3 ubiquitin-protein ligase CBL-B; RING finger protein 56

Application Instructions

Application Suggestion	Tested Application Dilution WB 1:500 - 1:2000
Application Note	* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control	Jurkat

Properties

Form	Liquid
Purification	Affinity purification with immunogen.
Buffer	PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative	0.02% Sodium azide
Stabilizer	50% Glycerol
Storage Instruction	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links	GeneID: 171136 Rat CBLB GeneID: 208650 Mouse CBLB GeneID: 868 Human CBLB Swiss-port # Q13191 Human E3 ubiquitin-protein ligase CBL-B Swiss-port # Q3TTA7 Mouse E3 ubiquitin-protein ligase CBL-B Swiss-port # Q8K4S7 Rat E3 ubiquitin-protein ligase CBL-B More
Gene Symbol	CBLB
Gene Full Name	Cbl proto-oncogene B, E3 ubiquitin protein ligase
Function	E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and transfers it to substrates, generally promoting their degradation by the proteasome. Negatively regulates TCR (T-cell receptor), BCR (B-cell receptor) and FCER1 (high affinity immunoglobulin epsilon receptor) signal transduction pathways. In naive T-cells, inhibits VAV1 activation upon TCR engagement and imposes a requirement for CD28 costimulation for proliferation and IL-2 production. Also acts by promoting PIK3R1/p85 ubiquitination, which impairs its recruitment to the TCR and subsequent activation. In activated T-cells, inhibits PLCG1 activation and calcium mobilization upon restimulation and promotes anergy. In B-cells, acts by ubiquitinating SYK and promoting its proteasomal degradation. Slightly promotes SRC ubiquitination. May be involved in EGFR ubiquitination and internalization. May be functionally coupled with the E2 ubiquitin-protein ligase UB2D3. [UniProt]
Calculated MW	109 kDa
PTM	Phosphorylated on tyrosine and serine residues upon TCR or BCR activation, and upon various types of cell stimulation. Auto-ubiquitinated upon EGF-mediated cell activation or upon T-cell costimulation by CD28; which promotes proteasomal degradation.

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