ARG65394
anti-CD116 antibody [4H1]
anti-CD116 antibody [4H1] for CyTOF®-candidate,Flow cytometry,IHC-Frozen sections,Immunoprecipitation,Western blot and Human
Developmental Biology antibody; Immune System antibody
Overview
| Product Description | Mouse Monoclonal antibody [4H1] recognizes CD116 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | CyTOF®-candidate, FACS, IHC-Fr, IP, WB |
| Specificity | The clone 4H1 recognizes human CD116, the GM-CSF receptor alpha subunit (approx. 80 kDa) expressed e.g. by neutrophils, eosinophils, monocytes and macrophages. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 4H1 |
| Isotype | IgG1 |
| Target Name | CD116 |
| Immunogen | CD116-transfected COS cells |
| Conjugation | Un-conjugated |
| Alternate Names | Granulocyte-macrophage colony-stimulating factor receptor subunit alpha; GMCSFR; CDw116; GM-CSF-R-alpha; CD antigen CD116; CSF2R; CD116; GMR-alpha; CSF2RAX; CSF2RAY; GMCSFR-alpha; GMR; SMDP4; CSF2RX; CSF2RY |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from cell culture supernatant by protein-A affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | TBS (pH 8.0) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P15509 Human Granulocyte-macrophage colony-stimulating factor receptor subunit alpha |
|---|---|
| Gene Symbol | CSF2RA |
| Gene Full Name | colony stimulating factor 2 receptor, alpha, low-affinity (granulocyte-macrophage) |
| Background | CD116 (GM-CSF R alpha) is the low affinity receptor for granulocyte-macrophage colony-stimulating factor (GM-CSF). CD116 heterodimerizes with CD131, the common beta chain subunit shared with IL-3 and IL5 receptors, to form the high affinity GM-CSF receptor. CD116 is expressed by myeloid cells including macrophages, neutrophils, eosinophils, dendritic cells, and their precursors, as well as on endothelial cells. It is being used as a specific marker of myeloid leukemias. |
| Function | Low affinity receptor for granulocyte-macrophage colony-stimulating factor. Transduces a signal that results in the proliferation, differentiation, and functional activation of hematopoietic cells. [UniProt] |
| Highlight | Related products: CD116 antibodies; CD116 ELISA Kits; Anti-Mouse IgG secondary antibodies; Related news: CyTOF-candidate Antibodies |
| Research Area | Developmental Biology antibody; Immune System antibody |
| Calculated MW | 46 kDa |
Images (2) Click the Picture to Zoom In
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ARG65394 anti-CD116 antibody [4H1] FACS image
Flow Cytometry: Human peripheral blood stained with ARG65394 anti-CD116 antibody [4H1] at 3 µg/ml dilution, followed by PE-conjugated Goat anti-Mouse antibody.
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ARG65394 anti-CD116 antibody [4H1] FACS image
Flow Cytometry: Separation of human monocytes (red-filled) from lymphocytes (black-dashed). Human peripheral whole blood stained with ARG65394 anti-CD116 antibody [4H1] at 3 µg/ml dilution, followed by PE-conjugated Goat anti-Mouse antibody.
Customer's Feedback
Average
Review for anti-CD116 antibody [4H1]
Application：WB
Sample：293T
Sample Loading Amount：20 µg
Primary Antibody Dilution Factor：1:300
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
