ARG22728
anti-CD11d antibody [CA11.8H2]
anti-CD11d antibody [CA11.8H2] for Flow cytometry,IHC-Frozen sections,Immunoprecipitation and Dog
Overview
| Product Description | Mouse Monoclonal antibody [CA11.8H2] recognizes CD11d This antibody recognizes Canine CD11d cell surface antigen, also known as integrin alpha d subunit. The alpha d integrin (CD11d) subunit is expressed as a heterodimer with beta 2 integrin (CD18). CD11d is expressed by a sub-population of macrophages, some CD8 positive T cells and by gamma/delta T cells in the splenic red pulp. Over expression of CD11d is noted in histiocytic disorders including hemophagocytic sarcoma, described as a proliferative disorder specifically of CD11+ macrophages (Moore. et al. 2006) with macrophages demonstrating marked erythrocytic phagocytosis. Previously, Canine histiocytic sarcoma were described as originating entirely from interstitial dendritic cells. Cells from animals with Canine hepatosplenic T cell lymphoma (HTSL) strongly express CD11d on malignant cells. Dogs normally demonstrate strong positivity for CD11d on large granular lymphocytes, macrophages and T cells in the splenic red pulp. CD11+ γ/δ T cells constitute ~30% red pulp T cells despite their rarity in peripheral blood, suggesting a splenic red pulp origin of HTSL (Fry et al. 2003). |
|---|---|
| Tested Reactivity | Dog |
| Tested Application | FACS, IHC-Fr, IP |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | CA11.8H2 |
| Isotype | IgG1 |
| Target Name | CD11d |
| Antigen Species | Dog |
| Immunogen | Affinity purified CD11 antigen from canine spleen. |
| Conjugation | Un-conjugated |
| Alternate Names | Integrin alpha-D; CD11 antigen-like family member D; ADB2; Leukointegrin alpha D; CD11D; CD antigen CD11d |
Application Instructions
| Application Suggestion |
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| Application Note | FACS: Use 10 µl of the suggested working dilution to label 10^6 cells or 100 µl whole blood. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS and 0.09% Sodium azide. |
| Preservative | 0.09% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | ITGAD |
|---|---|
| Gene Full Name | integrin, alpha D |
| Function | Integrin alpha-D/beta-2 is a receptor for ICAM3 and VCAM1. May play a role in the atherosclerotic process such as clearing lipoproteins from plaques and in phagocytosis of blood-borne pathogens, particulate matter, and senescent erythrocytes from the blood. [UniProt] |
| Calculated MW | 127 kDa |
Images (1) Click the Picture to Zoom In
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ARG22728 anti-CD11d antibody [CA11.8H2] FACS image
Flow Cytometry: Canine peripheral blood lymphocytes stained with ARG22728 anti-CD11d antibody [CA11.8H2] followed by Goat anti-Mouse IgG (H/L) (FITC).
