ARG42488
anti-CD15 antibody
anti-CD15 antibody for IHC-Formalin-fixed paraffin-embedded sections and Human,Rat
Overview
| Product Description | Mouse Monoclonal antibody recognizes CD15 |
|---|---|
| Tested Reactivity | Hu, Rat |
| Tested Application | IHC-P |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG |
| Target Name | CD15 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide of Human CD15. |
| Conjugation | Un-conjugated |
| Alternate Names | LeX; CD15; ELFT; FCT3A; FUTIV; SSEA-1; FUC-TIV; Alpha-(1,3)-fucosyltransferase 4; EC 2.4.1.-; ELAM-1 ligand fucosyltransferase; Fucosyltransferase 4; Fucosyltransferase IV; Fuc-TIV; FucT-IV; Galactoside 3-L-fucosyltransferase |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: 1) Heat mediated Sodium citrate buffer (pH 6.0) for 20 min, or 2) EDTA buffer (pH 8.0) at high pressure and temperature. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS (pH 7.4), 0.02% Sodium azide, 50% Glycerol and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol and 0.5% BSA |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | FUT4 |
| Gene Full Name | fucosyltransferase 4 (alpha (1,3) fucosyltransferase, myeloid-specific) |
| Background | The product of this gene transfers fucose to N-acetyllactosamine polysaccharides to generate fucosylated carbohydrate structures. It catalyzes the synthesis of the non-sialylated antigen, Lewis x (CD15). [provided by RefSeq, Jan 2009] |
| Function | May catalyze alpha-1,3 glycosidic linkages involved in the expression of Lewis X/SSEA-1 and VIM-2 antigens. [UniProt] |
| Cellular Localization | Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein. Note=Membrane-bound form in trans cisternae of Golgi. [UniProt] |
| Calculated MW | 59 kDa |
Images (4) Click the Picture to Zoom In
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ARG42488 anti-CD15 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human lung cancer tissue. Antigen Retrieval: Heat mediated Sodium citrate buffer (pH 6.0) for 20 min. The tissue section was stained with ARG42488 anti-CD15 antibody at 1:200 dilution, overnight at 4°C. Negative control was used by secondary antibody only.
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ARG42488 anti-CD15 antibody IHC image
Immunofluorescence: Human liver cancer tissue stained with ARG42488 anti-CD15 antibody (red) at 1:200 dilution, overnight at 4°C. DAPI (blue) for nuclear staining.
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ARG42488 anti-CD15 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat brain tissue stained with ARG42488 anti-CD15 antibody at 1:500 dilution.
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ARG42488 anti-CD15 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human stomach tissue. Antigen Retrieval: EDTA buffer (pH 8.0) at high pressure and temperature. The tissue section was stained with ARG42488 anti-CD15 antibody at 1:200 dilution, overnight at 4°C.
