ARG23422
anti-CD154 / CD40L antibody [TRAP-1]
anti-CD154 / CD40L antibody [TRAP-1] for Flow cytometry and Human
Overview
| Product Description | Mouse Monoclonal antibody [TRAP-1] recognizes CD154 / CD40L Mouse anti Human CD154 antibody, clone TRAP-1 recognises the human CD40 ligand, also known as CD154, TNF-related activation protein (TRAP) or T-cell antigen Gp39. CD154 is a 261 amino acid ~ 32kDa single pass, type-1 transmembrane glycoprotein (UniProt: P29965). CD154 is expressed on activated T lymphocytes, predominantly CD4 +ve and also on some basophils and mast cells.Mouse anti Human CD154 antibody, clone TRAP-1 binds to CD154 at an epitope distinct from the CD40 binding site (Kroczek et al. 1994). |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | TRAP-1 |
| Isotype | IgG1 |
| Target Name | CD154 / CD40L |
| Antigen Species | Human |
| Immunogen | Mouse myeloma cell line transfected with human CD40L (CD154) |
| Conjugation | Un-conjugated |
| Alternate Names | TNFSF5; IMD3; T-cell antigen Gp39; HIGM1; CD40-L; gp39; CD40 ligand; Tumor necrosis factor ligand superfamily member 5; CD40L; CD154; TRAP; CD antigen CD154; hCD40L; IGM; T-BAM; TNF-related activation protein |
Application Instructions
| Application Suggestion |
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| Application Note | FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS, 0.09% Sodium azide and 0.1% BSA. |
| Preservative | 0.09% Sodium azide |
| Stabilizer | 0.1% BSA |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CD40LG |
| Gene Full Name | CD40 ligand |
| Background | The protein encoded by this gene is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome. [provided by RefSeq, Jul 2008] |
| Function | Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching. Release of soluble CD40L from platelets is partially regulated by GP IIb/IIIa, actin polymerization, and an matrix metalloproteinases (MMP) inhibitor-sensitive pathway. [UniProt] |
| Calculated MW | 29 kDa |
| PTM | The soluble form derives from the membrane form by proteolytic processing. N-linked glycan is a mixture of high mannose and complex type. Glycan structure does not influence binding affinity to CD40. Not O-glycosylated. [UniProt] |
