ARG62749
anti-CD177 antibody [MEM-166] (FITC)
anti-CD177 antibody [MEM-166] (FITC) for Flow cytometry and Human,Primates
Immune System antibody
Overview
| Product Description | FITC-conjugated Mouse Monoclonal antibody [MEM-166] recognizes CD177 |
|---|---|
| Tested Reactivity | Hu, NHuPrm |
| Tested Application | FACS |
| Specificity | The clone MEM-166 reacts with CD177 (Neutrophil specific antigen 1), a 60 kDa GPI-linked cell surface glycoprotein of uPAR family, expressed on granulocytes and in bone marrow early erythroblasts, megakaryocytes, promyelocytes and myelocytes. HLDA VI; WS Code M M17 HLDA VI; WS Code BP 309 |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | MEM-166 |
| Isotype | IgG1 |
| Target Name | CD177 |
| Antigen Species | Human |
| Immunogen | Human granulocytes |
| Conjugation | FITC |
| Alternate Names | NB1 glycoprotein; PRV-1; PRV1; NB1; CD177 antigen; Human neutrophil alloantigen 2a; NB1 GP; HNA2A; Polycythemia rubra vera protein 1; CD antigen CD177; HNA-2a |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification Note | The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use. No reconstitution is necessary. |
| Buffer | PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA |
| Preservative | 15 mM Sodium azide |
| Stabilizer | 0.2% (w/v) high-grade protease free BSA |
| Storage Instruction | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CD177 |
| Gene Full Name | CD177 molecule |
| Background | CD177 (NB1/HNA-2a and PRV-1 form) is a GPI-anchored glycoprotein present mainly on neutrophils. Its plasma membrane expression is increased during pregnancy and and inflammation or after G-CSF application. Ligand of CD177 has been identified as CD31 (PECAM-1). CD177 participates in neutrophil transmigration and seems to be also a pro-proliferative molecule. The antibodies against CD177 can be involved in neonatal alloimmune neutropenia (NAN). |
| Research Area | Immune System antibody |
| Calculated MW | 46 kDa |
| PTM | N-glycosylated. A soluble form may also be produced by proteolytic cleavage at the cell surface (shedding). |
Images (2) Click the Picture to Zoom In
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ARG62749 anti-CD177 antibody [MEM-166] (FITC) FACS image
Flow Cytometry: Human peripheral whole blood stained with ARG62749 anti-CD177 antibody [MEM-166] (FITC) (20 µl reagent / 100 µl of peripheral whole blood).
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ARG62749 anti-CD177 antibody [MEM-166] (FITC) FACS image
Flow Cytometry: Separation of human CD177 positive neutrophil granulocytes (red-filled) from lymphocytes (black-dashed). Human peripheral whole blood stained with ARG62749 anti-CD177 antibody [MEM-166] (FITC) (20 µl reagent / 100 µl of peripheral whole blood).
Clone References
Differential expression of granulopoiesis related genes in neutrophil subsets distinguished by membrane expression of CD177.
WB, FACS / Human
The Thr224Asn mutation in the VPS45 gene is associated with the congenital neutropenia and primary myelofibrosis of infancy.
IHC-P / Human
Isolation of primitive endoderm, mesoderm, vascular endothelial and trophoblast progenitors from human pluripotent stem cells.
Decreased CXCR1 and CXCR2 expression on neutrophils in anti-neutrophil cytoplasmic autoantibody-associated vasculitides potentially increases neutrophil adhesion and impairs migration.
FACS / Human
CXCR3-dependent accumulation and activation of perivascular macrophages is necessary for homeostatic arterial remodeling to hemodynamic stresses.
FACS / Human
