ARG62785
anti-CD25 antibody [MEM-140]
anti-CD25 antibody [MEM-140] for Flow cytometry,Immunoprecipitation and Human
Immune System antibody; Pre-B Cell Marker antibody
Overview
| Product Description | Mouse Monoclonal antibody [MEM-140] recognizes CD25 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, IP |
| Specificity | The clone MEM-140 reacts with CD25 (Interleukin-2 receptor alpha chain), a 55 kDa type I transmembrane glycoprotein expressed on activated B and T lymphocytes, activated monocytes/macrophages and on CD4+ T lymphocytes (T regulatory cells); it is lost on resting B and T lymphocytes. HLDA VI; WS Code C C-54 |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | MEM-140 |
| Isotype | IgM |
| Target Name | CD25 |
| Immunogen | PHA-activated peripheral blood leucocytes |
| Conjugation | Un-conjugated |
| Alternate Names | IL-2-RA; IL-2 receptor subunit alpha; CD25; TCGFR; TAC antigen; IL2R; CD antigen CD25; Interleukin-2 receptor subunit alpha; IL-2R subunit alpha; p55; IL2-RA; IDDM10 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | IP: Excellent for immunoisolation of CD25+cells. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from ascites by thiophilic adsorption-affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | TBS (pH 8.0) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P01589 Human Interleukin-2 receptor subunit alpha |
|---|---|
| Gene Symbol | IL2RA |
| Gene Full Name | interleukin 2 receptor, alpha |
| Background | CD25 (IL2Ralpha, Tac) is a ligand-binding alpha subunit of interleukin 2 receptor (IL2R). Together with beta and gamma subunit CD25 constitues the high affinity IL2R, whereas CD25 alone serves as the low affinity IL2R. CD25 expression rapidly increases upon T cell activation. The 55 kDa CD25 molecule is enzymatically cleaved and shed from the cell surface as a soluble 45 kDa s-Tac, whose concentration in serum can be used as a marker of T cell activation. Expression of CD25 indicates the neoplastic phenotype of mast cells. Humanized anti CD25 antibodies represent a useful tool to reduce the incidence of allograft rejection as well as the severity of graft versus host reaction, and radioimmunoconjugates of anti-CD25 antibodies can be used against CD25 expressing lymphomas. |
| Function | Receptor for interleukin-2. [UniProt] |
| Research Area | Immune System antibody; Pre-B Cell Marker antibody |
| Calculated MW | 31 kDa |
Images (1) Click the Picture to Zoom In
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ARG62785 anti-CD25 antibody [MEM-140] FACS image
Flow Cytometry: Unstained (blue) and stained (red) PHA-stimulated human peripheral blood mononclear cells. Cells were stained with ARG62785 anti-CD25 antibody [MEM-140] at 0.6 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.
