ARG54262
anti-CD266 / TWEAK R antibody [ITEM-4] (PE)
anti-CD266 / TWEAK R antibody [ITEM-4] (PE) for Flow cytometry and Human,Mouse
Cancer antibody; Cell Biology and Cellular Response antibody; Signaling Transduction antibody
Overview
| Product Description | PE-conjugated Mouse Monoclonal antibody [ITEM-4] recognizes CD266 / TWEAK R |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | FACS |
| Specificity | The clone ITEM-4 recognizes CD266 / TWEAK R, a TNFR superfamily receptor for CD255 / TWEAK, a TNF-like weak inducer of apoptosis. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | ITEM-4 |
| Isotype | IgG2b |
| Target Name | CD266 / TWEAK R |
| Antigen Species | Human |
| Immunogen | human CD266-transfected P815 cells |
| Conjugation | PE |
| Alternate Names | Tumor necrosis factor receptor superfamily member 12A; FGF-inducible 14; CD266; Fibroblast growth factor-inducible immediate-early response protein 14; TWEAKR; TweakR; CD antigen CD266; Tweak-receptor; FN14 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification Note | The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography. |
| Buffer | PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA |
| Preservative | 15 mM Sodium azide |
| Stabilizer | 0.2% (w/v) high-grade protease free BSA |
| Storage Instruction | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q9CR75 Mouse Tumor necrosis factor receptor superfamily member 12A Swiss-port # Q9NP84 Human Tumor necrosis factor receptor superfamily member 12A |
|---|---|
| Gene Symbol | TNFRSF12A |
| Gene Full Name | tumor necrosis factor receptor superfamily, member 12A |
| Background | CD266 / TWEAK R (TNFRSF12A), also known as FN14 (fibroblast growth factor-inducible 14) is a receptor for CD255 / TWEAK, the TNF-like weak inducer of apoptosis. CD266 is expressed on endothelial cells, as well as on some cancer tissues, and plays a role in CD255-induced endothelial cell migration, proliferation, and angiogenesis. The CD255-CD266 interaction, or antibody-mediated triggering of CD266 is also able to induce apoptosis and necrosis in CD266-positive cells (including tumor cells), which might have therapeutic potential. |
| Function | Receptor for TNFSF12/TWEAK. Weak inducer of apoptosis in some cell types. Promotes angiogenesis and the proliferation of endothelial cells. May modulate cellular adhesion to matrix proteins. [UniProt] |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Signaling Transduction antibody |
| Calculated MW | 14 kDa |
Images (1) Click the Picture to Zoom In
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ARG54262 anti-CD266 / TWEAK R antibody [ITEM-4] (PE) FACS image
Flow Cytometry: Separation of unstained (black-dashed) and stained (red-filled) CD266 transfected L51784 cells. Cells were stained with ARG54262 anti-CD266 / TWEAK R antibody [ITEM-4] (PE) at 0.19 µg/ml dilution.
Clone References
TWEAK mediates signal transduction and differentiation of RAW264.7 cells in the absence of Fn14/TweakR. Evidence for a second TWEAK receptor.
