ARG62793
anti-CD27 antibody [LT27]
anti-CD27 antibody [LT27] for Flow cytometry and Human
Cancer antibody; Developmental Biology antibody; Immune System antibody
Overview
| Product Description | Mouse Monoclonal antibody [LT27] recognizes CD27 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS |
| Specificity | The clone LT27 reacts with CD27 (T14), a 50-55 kDa type I transmembrane glycoprotein (member of the TNF-receptor superfamily) expressed on medullary thymocytes, peripheral T lymphocytes, some B lymphocytes and NK cells. HLDA V; WS Code T T-CD27.01 |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | LT27 |
| Isotype | IgG2a |
| Target Name | CD27 |
| Antigen Species | Human |
| Immunogen | Human peripheral blood lymphocytes |
| Conjugation | Un-conjugated |
| Alternate Names | T-cell activation antigen CD27; Tp55; CD antigen CD27; CD27 antigen; S152. LPFS2; T14; S152; Tumor necrosis factor receptor superfamily member 7; CD27L receptor; TNFRSF7 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from ascites by precipitation methods and ion exchange chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CD27 |
| Gene Full Name | CD27 molecule |
| Background | CD27 is a transmembrane 55 kDa protein of the nerve growth factor-receptor family, expressed as a disulfide-linked homodimer on mature thymocytes, peripheral blood T cells and a subpopulation of B cells. Activation of T cells via TCR-CD3 complex results in upregulation of CD27 expression on the plasma membrane as well as in the release of its soluble 28-32 kDa form, sCD27, detected in the plasma, urine or spinal fluid. This sCD27 is an important prognostic marker of acute and chronic B cell malignancies. RgpA, a cystein proteinase, although activating T cells through the protease-activated receptors (PARs), degradates CD27 and counteracts T cell activation mediated by CD27 and its ligand CD70. |
| Function | Receptor for CD70/CD27L. May play a role in survival of activated T-cells. May play a role in apoptosis through association with SIVA1. [UniProt] |
| Research Area | Cancer antibody; Developmental Biology antibody; Immune System antibody |
| Calculated MW | 29 kDa |
| PTM | Phosphorylated. O-glycosylated with core 1 or possibly core 8 glycans. |
Clone References
Reduced expression of adhesion molecules and cell signaling receptors by chronic lymphocytic leukemia cells with 11q deletion.
