ARG22661
anti-CD301 antibody [ER-MP23]
anti-CD301 antibody [ER-MP23] for Flow cytometry,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections and Mouse
Overview
| Product Description | Rat Monoclonal antibody [ER-MP23] recognizes CD301 This antibody recognizes murine CD301, a ~38 kDa cell surface protein, otherwise known as macrophage galactose N-acetylgalactosamine lectin (MGL) or dendritic cell asialoglycoprotein (DC-ASGPR). |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | FACS, ICC/IF, IHC-Fr, IHC-P |
| Host | Rat |
| Clonality | Monoclonal |
| Clone | ER-MP23 |
| Isotype | IgG2a |
| Target Name | CD301 |
| Antigen Species | Mouse |
| Immunogen | Balb/c macrophage precursor cell hybrids. |
| Conjugation | Un-conjugated |
| Alternate Names | CD301; CD antigen CD301; Macrophage lectin 2; C-type lectin superfamily member 14; HML2; HML; CLECSF14; CLECSF13; C-type lectin domain family 10 member A; MGL |
Application Instructions
| Application Suggestion |
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| Application Note | FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl. IHC-P: Antigen Retrieval: Boil tissue section in Citrate buffer (pH 6.0) * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS and 0.09% Sodium azide. |
| Preservative | 0.09% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P49300 Mouse C-type lectin domain family 10 member A |
|---|---|
| Gene Symbol | Clec10a |
| Gene Full Name | C-type lectin domain family 10, member A |
| Background | This gene encodes a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. Members of this family share a common protein fold and have diverse functions, such as cell adhesion, cell-cell signalling, glycoprotein turnover, and roles in inflammation and immune response. The encoded type 2 transmembrane protein may function as a cell surface antigen. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008] |
| Function | Probable role in regulating adaptive and innate immune responses. Binds in a calcium-dependent manner to terminal galactose and N-acetylgalactosamine units, linked to serine or threonine. These sugar moieties are known as Tn-Ag and are expressed in a variety of carcinoma cells. [UniProt] |
| Calculated MW | 35 kDa |
Images (3) Click the Picture to Zoom In
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ARG22661 anti-CD301 antibody [ER-MP23] IHC-P image
Immunohistochemistry: Immunoperoxidase staining of a Mouse lymph node stained with ARG22661 anti-CD301 antibody [ER-MP23]. (Low power).
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ARG22661 anti-CD301 antibody [ER-MP23] IHC-P image
Immunohistochemistry: Immunoperoxidase staining of a Mouse lymph node stained with ARG22661 anti-CD301 antibody [ER-MP23]. (Medium power).
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ARG22661 anti-CD301 antibody [ER-MP23] IHC-P image
Immunohistochemistry: Immunoperoxidase staining of a Mouse lymph node stained with ARG22661 anti-CD301 antibody [ER-MP23]. (High power).
