ARG65378
anti-CD314 / NKG2D antibody [1D11]
anti-CD314 / NKG2D antibody [1D11] for Flow cytometry,Functional study,IHC-Frozen sections,Immunoprecipitation and Human
Immune System antibody
Overview
| Product Description | Mouse Monoclonal antibody [1D11] recognizes CD314 / NKG2D |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, FuncSt, IHC-Fr, IP |
| Specificity | The clone 1D11 recognizes CD314 / NKG2D, a 42 kDa C-type lectin-like activating receptor expressed by NK cells, gamma/delta T cells, and CD8+ T cells. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 1D11 |
| Isotype | IgG1 |
| Target Name | CD314 / NKG2D |
| Immunogen | NKL cell line |
| Conjugation | Un-conjugated |
| Alternate Names | NKG2-D-activating NK receptor; CD antigen CD314; D6H12S2489E; NK cell receptor D; NKG2-D type II integral membrane protein; NKG2-D; Killer cell lectin-like receptor subfamily K member 1; Nkg2d |
Application Instructions
| Application Suggestion |
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| Application Note | Functional studies: Blocking of ligand binding. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from cell culture supernatant by protein-A affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P26718 Human NKG2-D type II integral membrane protein |
|---|---|
| Gene Symbol | KLRK1 |
| Gene Full Name | killer cell lectin-like receptor subfamily K, member 1 |
| Background | CD314, also known as NKG2D (natural killer receptor G2D) or KLRK1 (killer cell lectin-like receptor subfamily K, member 1), is a homodimeric C-type lectin-like activating receptor and costimulator with type II membrane orientation (C teminus extracellular). CD314 homodimers are associated with DAP10, a membrane adaptor protein that signals similar to CD28 by recruitment of phosphatidylinositol 3-kinase. Engagement of CD314 amplifies antigen-specific T cell responses in CD314-positive T cell populations. In NK cells, CD314 is a primary activating receptor. As CD314 ligands the MHC class-I chain-related proteins A and B (MICA, MICB) and UL16-binding proteins (ULBPs) have been identified. |
| Function | Function as an activating and costimulatory receptor involved in immunosurveillance upon binding to various cellular stress-inducible ligands displayed at the surface of autologous tumor cells and virus-infected cells. Provides both stimulatory and costimulatory innate immune responses on activated killer (NK) cells, leading to cytotoxic activity. Acts as a costimulatory receptor for T-cell receptor (TCR) in CD8(+) T-cell-mediated adaptive immune responses by amplifying T-cell activation. Stimulates perforin-mediated elimination of ligand-expressing tumor cells. Signaling involves calcium influx, culminating in the expression of TNF-alpha. Participates in NK cell-mediated bone marrow graft rejection. May play a regulatory role in differentiation and survival of NK cells. Binds to ligands belonging to various subfamilies of MHC class I-related glycoproteins including MICA, MICB, RAET1E, RAET1G, ULBP1, ULBP2, ULBP3 (ULBP2>ULBP1>ULBP3) and ULBP4. [UniProt] |
| Research Area | Immune System antibody |
| Calculated MW | 25 kDa |
Images (2) Click the Picture to Zoom In
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ARG65378 anti-CD314 / NKG2D antibody [1D11] FACS image
Flow Cytometry: Human peripheral blood stained with ARG65378 anti-CD314 / NKG2D antibody [1D11] at 4 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.
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ARG65378 anti-CD314 / NKG2D antibody [1D11] FACS image
Flow Cytometry: Separation of human CD314 / NKG2D positive lymphocytes (red-filled) from CD314 / NKG2D negative lymphocytes (black-dashed). Human peripheral whole blood stained with ARG65378 anti-CD314 / NKG2D antibody [1D11] at 4 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.
Clone References
In vitro Natural Killer Cell Immunotherapy for Medulloblastoma.
FACS / Human
Rescue of impaired NK cell activity in hodgkin lymphoma with bispecific antibodies in vitro and in patients.
FACS / Human
Inhibitory signaling blocks activating receptor clustering and induces cytoskeletal retraction in natural killer cells.
FuncSt / Human
Genome-wide association study in alopecia areata implicates both innate and adaptive immunity.
IHC-Fr / Human
