ARG62842

anti-CD41 antibody [MEM-06] (FITC)

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100 tests

399.00

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Overview

Product Description	FITC-conjugated Mouse Monoclonal antibody [MEM-06] recognizes CD41
Tested Reactivity	Hu
Tested Application	FACS
Specificity	The clone MEM-06 reacts with CD41 (GPIIb), a transmembrane glycoprotein (integrin family) composed of two chains GPIIb alpha (heavy chain; 120 kDa) and GPIIb beta (light chain; 23 kDa). CD41 is mainly expressed on platelets and megakaryocytes.
Host	Mouse
Clonality	Monoclonal
Clone	MEM-06
Isotype	IgG1
Target Name	CD41
Immunogen	Leukocytes of patient suffering from LGL-type leukaemia.
Conjugation	FITC
Alternate Names	GTA; GT; GPalpha IIb; PPP1R93; CD41; BDPLT2; BDPLT16; GP2B; Integrin alpha-IIb; GPIIb; Platelet membrane glycoprotein IIb; HPA3; CD antigen CD41; CD41B

Application Instructions

Application Suggestion

Tested Application	Dilution
FACS	20 µl / 10^6 cells

Application Note

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form	Liquid
Purification Note	The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use. No reconstitution is necessary.
Buffer	PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA
Preservative	15 mM Sodium azide
Stabilizer	0.2% (w/v) high-grade protease free BSA
Storage Instruction	Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links	GeneID: 3674 Human ITGA2B Swiss-port # P08514 Human Integrin alpha-IIb
Gene Symbol	ITGA2B
Gene Full Name	integrin, alpha 2b (platelet glycoprotein IIb of IIb/IIIa complex, antigen CD41)
Background	CD41 (platelet glycoprotein IIb) is composed of two subunits (120 kDa a, alpha and 23 kDa b, beta) that interact with CD61 in the presence of calcium to form a functional adhesive protein receptor. Upon blood vessel damage, this receptor binds to a variety of proteins including von Willebrand factor, fibrinogen, fibronectin and vitronectin. CD41 is mainly expressed on megakaryocyte-platelet lineage, but generally belongs to the antigens that are expressed during early stages of hematopoietic differentiation.
Function	Integrin alpha-IIb/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. It recognizes the sequence R-G-D in a wide array of ligands. It recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial cell surface. [UniProt]
Research Area	Cell Biology and Cellular Response antibody; Developmental Biology antibody; Immune System antibody; Signaling Transduction antibody
Calculated MW	113 kDa

Images (2) Click the Picture to Zoom In

ARG62842 anti-CD41 antibody [MEM-06] (FITC) FACS image

Flow Cytometry: Human peripheral whole blood stained with ARG62842 anti-CD41 antibody [MEM-06] (FITC) (20 µl reagent / 100 µl of peripheral whole blood).
ARG62842 anti-CD41 antibody [MEM-06] (FITC) FACS image

Flow Cytometry: Separation of human CD41 positive thrombocytes (red-filled) from CD41 negative lymphocytes (black-dashed). Human peripheral whole blood stained with ARG62842 anti-CD41 antibody [MEM-06] (FITC) (20 µl reagent / 100 µl of peripheral whole blood).