ARG65621
anti-CD41 antibody [MWReg30] (low endotoxin)
anti-CD41 antibody [MWReg30] (low endotoxin) for ELISA,Flow cytometry,Functional study,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation and Mouse
Cell Biology and Cellular Response antibody; Developmental Biology antibody; Immune System antibody; Signaling Transduction antibody
Overview
| Product Description | Low endotoxin Rat Monoclonal antibody [MWReg30] recognizes CD41 (low endotoxin) |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | ELISA, FACS, FuncSt, IHC-Fr, IHC-P, IP |
| Specificity | This antibody recognizes CD41 (GPIIb), a transmembrane glycoprotein (integrin family) composed of two chains GPIIb alpha (heavy chain; 120 kDa) and GPIIb beta (light chain; 23 kDa). CD41 is mainly expressed on platelets and megakaryocytes. |
| Host | Rat |
| Clonality | Monoclonal |
| Clone | MWReg30 |
| Isotype | IgG1 |
| Target Name | CD41 |
| Antigen Species | Mouse |
| Immunogen | Murine platelets |
| Conjugation | Un-conjugated |
| Alternate Names | GTA; GT; GPalpha IIb; PPP1R93; CD41; BDPLT2; BDPLT16; GP2B; Integrin alpha-IIb; GPIIb; Platelet membrane glycoprotein IIb; HPA3; CD antigen CD41; CD41B |
Application Instructions
| Application Suggestion |
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| Application Note | Functional application: Platelet depletion. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Purification Note | 0.2 µm filter sterilized. Endotoxin level is <0.01 EU/µg of the protein. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | Itga2b |
| Gene Full Name | integrin alpha 2b |
| Background | ITGA2B encodes integrin alpha chain 2b. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. Alpha chain 2b undergoes post-translational cleavage to yield disulfide-linked light and heavy chains that join with beta 3 to form a fibronectin receptor expressed in platelets that plays a crucial role in coagulation. Mutations that interfere with this role result in thrombasthenia. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling. [provided by RefSeq, Jul 2008] |
| Function | Integrin alpha-IIb/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. It recognizes the sequence R-G-D in a wide array of ligands. It recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial cell surface. [UniProt] |
| Research Area | Cell Biology and Cellular Response antibody; Developmental Biology antibody; Immune System antibody; Signaling Transduction antibody |
| Calculated MW | 113 kDa |
Clone References
Homing and adhesion patterns determine the cellular composition of the bone marrow plasma cell niche.
Micronutrients attenuate progression of prostate cancer by elevating the endogenous inhibitor of angiogenesis, platelet factor-4.
IHC-P / Mouse
ERG is a megakaryocytic oncogene.
FACS / Human
Characterization of purified intraembryonic hematopoietic stem cells as a tool to define their site of origin.
Expression of CD41 on hematopoietic progenitors derived from embryonic hematopoietic cells.
