ARG62874
anti-CD48 antibody [MEM-102]
anti-CD48 antibody [MEM-102] for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Primates
Immune System antibody
Overview
| Product Description | Mouse Monoclonal antibody [MEM-102] recognizes CD48 |
|---|---|
| Tested Reactivity | Hu, NHuPrm |
| Tested Application | FACS, IHC-P, IP, WB |
| Specificity | The clone MEM-102 reacts with CD48 antigen (Blast-1), a 40-47 kDa GPI-anchored membrane protein (immunoglobulin supergene family) widely expressed on hematopoietic cells; it is negative on granulocytes, platelets and erythrocytes. HLDA V; WS Code AS S014 |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | MEM-102 |
| Isotype | IgG1 |
| Target Name | CD48 |
| Antigen Species | Human |
| Immunogen | Raji human Burkitt's lymphoma cell line |
| Conjugation | Un-conjugated |
| Alternate Names | Leukocyte antigen MEM-102; B-lymphocyte activation marker BLAST-1; CD48 antigen; BCM1 surface antigen; CD antigen CD48; TCT.1; BLAST1; BCM1; MEM-102; mCD48; Signaling lymphocytic activation molecule 2; BLAST; hCD48; SLAMF2; SLAM family member 2 |
Application Instructions
| Application Suggestion |
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| Application Note | WB: Under non-reducing condition. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from ascites by protein-A affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CD48 |
| Gene Full Name | CD48 molecule |
| Background | CD48 (Blast-1) belongs to the CD2 subset of the Ig superfamily, which includes CD2, CD2F-10, CD58, CD84, CD150, CD229, CD244 and others. These molecules bind to the same or another members of their family, thus mediate homotypic or heterotypic adhesion. CD48 is a GPI-anchored protein broadly expressed on hematopoietic cells and serves as a high affinity ligand for 2B4 and low affinity ligand for CD2. 2B4-CD48 interaction among NK cells and NK-T cells regulates cell proliferation. Signaling through CD48 results in eosinophil activation and CD48 expression is increased in several infectious diseases. |
| Function | Ligand for CD2. Might facilitate interaction between activated lymphocytes. Probably involved in regulating T-cell activation. [UniProt] |
| Research Area | Immune System antibody |
| Calculated MW | 28 kDa |
Clone References
Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement.
Signal transduction via glycosyl phosphatidylinositol-anchored proteins in T cells is inhibited by lowering cellular cholesterol.
The human leucocyte antigen CD48 (MEM-102) is closely related to the activation marker Blast-1.
