ARG66751
anti-CD5 antibody [SQab20184]
anti-CD5 antibody [SQab20184] for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Overview
| Product Description | Recombinant Rabbit Monoclonal antibody [SQab20184] recognizes CD5 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | SQab20184 |
| Isotype | IgG |
| Target Name | CD5 |
| Antigen Species | Human |
| Immunogen | Recombinant full length protein of Human CD5 (extracellular). The immunogen contains the specific extracellular domain (D32-P372). |
| Conjugation | Un-conjugated |
| Alternate Names | CD antigen CD5; Lymphocyte antigen T1/Leu-1; LEU1; T-cell surface glycoprotein CD5; T1 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Positive Control | WB: Jurkat IHC-P: Appendix tissue |
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| Observed Size | ~ 52 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA. |
| Preservative | 0.01% Sodium azide |
| Stabilizer | 40% Glycerol and 0.05% BSA |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CD5 |
| Gene Full Name | CD5 molecule |
| Background | This gene encodes a member of the scavenger receptor cysteine-rich (SRCR) superfamily. Members of this family are secreted or membrane-anchored proteins mainly found in cells associated with the immune system. This protein is a type-I transmembrane glycoprotein found on the surface of thymocytes, T lymphocytes and a subset of B lymphocytes. The encoded protein contains three SRCR domains and may act as a receptor to regulate T-cell proliferation. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2016] |
| Function | May act as a receptor in regulating T-cell proliferation. [UniProt] |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein. [UniProt] |
| Calculated MW | 55 kDa |
| PTM | Phosphorylated on tyrosine residues by LYN; this creates binding sites for PTPN6/SHP-1. [UniProt] |
Images (2) Click the Picture to Zoom In
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ARG66751 anti-CD5 antibody [SQab20184] WB image (Customer's Feedback)
Western blot: 10 µg of Jurkat cell lysate stained with ARG66751 anti-CD5 antibody [SQab20184] at 1:1000 dilution.
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ARG66751 anti-CD5 antibody [SQab20184] IHC-P image
Immunohistochemistry: Formalin/PFA-fixed and paraffin-embedded Human appendix tissue. Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0). The tissue section was stained with ARG66751 anti-CD5 antibody [SQab20184] at 18°C - 25°C for 30 minutes.
Customer's Feedback
Excellent
Review for anti-CD5 antibody [SQab20184]
Application：WB
Sample：Jurkat
Sample Loading Amount：10 µg
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
