ARG62916
anti-CD69 antibody [FN50]
anti-CD69 antibody [FN50] for CyTOF®-candidate,Flow cytometry,Functional study,IHC-Frozen sections and Human
Developmental Biology antibody; Immune System antibody
Overview
| Product Description | Mouse Monoclonal antibody [FN50] recognizes CD69 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | CyTOF®-candidate, FACS, FuncSt, IHC-Fr |
| Specificity | The clone FN50 recognizes CD69, an lymphocyte early activation marker. HLDA IV; WS Code A 91 |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | FN50 |
| Isotype | IgG1 |
| Target Name | CD69 |
| Antigen Species | Human |
| Immunogen | anti-µ-stimulated human B lymphocytes |
| Conjugation | Un-conjugated |
| Alternate Names | GP32/28; Activation inducer molecule; MLR-3; BL-AC/P26; Leukocyte surface antigen Leu-23; AIM; Early activation antigen CD69; Early T-cell activation antigen p60; EA1; CD antigen CD69; CLEC2C; C-type lectin domain family 2 member C |
Application Instructions
| Application Suggestion |
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| Application Note | Functional studies: When stimulating peripheral blood T cells, clone FN50 together with TPA synergistically increases cell volume and RNA/DNA synthesis. Addition of FN50 to stimulated peripheral blood B cells has similar, but weaker effect. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from cell culture supernatant by protein-A affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CD69 |
| Gene Full Name | CD69 molecule |
| Background | CD69 (C-type lectin domain family 2 C, CLEC2C, also known as AIM) is one of the earliest inducible cell surface molecules acquired during leukocyte activation. This glycoprotein serves as a lectin-type receptor in lymphocytes, NK cells and platelets; it is involved in lymphocyte proliferation. CD69 expression is counteracted on T cells in the AIDS stage of HIV infection, and may be also predictive for clinical response to chemoimmunotherapy. |
| Function | Involved in lymphocyte proliferation and functions as a signal transmitting receptor in lymphocytes, natural killer (NK) cells, and platelets. [UniProt] |
| Highlight | Related products: CD69 antibodies; Anti-Mouse IgG secondary antibodies; Related news: CyTOF-candidate Antibodies |
| Research Area | Developmental Biology antibody; Immune System antibody |
| Calculated MW | 23 kDa |
| PTM | Constitutive Ser/Thr phosphorylation in both mature thymocytes and activated T-lymphocytes. |
Images (1) Click the Picture to Zoom In
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ARG62916 anti-CD69 antibody [FN50] FACS image
Flow Cytometry: Human PHA-activated peripheral blood stained with ARG62916 anti-CD69 antibody [FN50], followed by incubation with APC labelled Goat anti-Mouse secondary antibody.
Clone References
CD98hc (SLC3A2) interaction with beta 1 integrins is required for transformation.
Multiple dimeric forms of human CD69 result from differential addition of N-glycans to typical (Asn-X-Ser/Thr) and atypical (Asn-X-cys) glycosylation motifs.
